新疆维、汉族高尿酸血症痰浊阻滞证与ABCG2多态性关系的研究

本课题组前期关于高尿酸血症流行病学调查结果表明，新疆地区维吾尔族以高脂饮食为主，但高尿酸血症患病率却低于汉族。因子分析结果，两民族中医证候构成不同，汉族人群痰浊阻滞证较突出，维吾尔族人群以脾肾两虚为主。上述差异除与饮食相关外，可能与尿酸代谢环节基因多态性相关。本项目拟通过等位基因特异性多重PCR技术、聚合酶链式反应一限制性片段长度多态性(PCR-RFLP)等技术，检测维吾尔族、汉族高尿酸血症痰浊阻滞证和非痰浊阻滞证患者的ABCG2基因单核苷酸多态性，了解维吾尔族、汉族ABCG2基因的分布特点，分析不同民族痰浊阻滞证与ABCG2基因的关联关系，探索不同民族高尿酸血症的分子遗传学机制，对今后开展预防、治疗以及药物研究、筛选治疗靶点基因等提供科学依据。

The early epidemiological survey of hyperuricemia in this project indicated that, Uygur people's diet is mainly high-fat diet, but hyperuricemia prevalence rate among them is lower than the Han. Results of factor analysis show that, TCM syndromes of these two nationalities are different, i.e. phlegm stasis syndrome is more prominent among the Han people while deficiency of spleen and kidney among the Uyghur people. Besides the diet habit, the above differences are most likely related to uric acid metabolism gene polymorphisms. By applying the technology of allele-specific multiplex PCR and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), the project intends to detect the ABCG2 genetic single nucleotide polymorphism of patients of hyperuricemia with and without phlegm stasis syndrome among Uygur and Han people, to learn the characteristics of distribution for the ABCG2 gene among them, to analyze the relationship of phlegm stasis syndrome and ABCG2 gene among different ethnic groups. The exploration on the molecular genetic mechanism of hyperuricemia between different ethnic groups is expected to provide a scientific basis for future prevention, treatment, and drug research, screening the target gene.