课题组关于云南特色药用植物灯盏花主要活性成分-黄酮类化合物灯盏花乙素(SCU)及其衍生物的前期研究提示：SCU 及其衍生物能拮抗高血压及缺血再灌注诱导的血管损伤（VD），改善其血管舒张及收缩（血管舒缩）功能，且与调控血管舒张的下游通路PKG/VASP(cGMP dependent protein kinase/vasodilator stimulated phosphoprotein)密切相关。本项目拟采用Wire Myograph 及分子生物学等技术，从动物、离体血管、细胞分子三个层次，证明SCU及其衍生物拮抗糖尿病合并高脂血症导致的VD，揭示其改善血管舒缩功能，与其调控血管舒张的上游通路NOS/GC/PKG（NO synthase/Guanylyl cyclase/PKG）密切相关。通过深入研究，揭示SCU及其衍生物拮抗血管VD的分子机制，对其用于防治多种疾病诱导的血管VD,提供依据。

Scutellarin（SCU）is a major active flavanoid compound from Yunnan characteristic native plant of Erigeron breviscapus (Vant.) Hand.-mazz. Our previous studies suggested that SCU and it’s derivative attenuated vascular dysfunction (VD) induced by hypertension and ischemia reperfusion, improved vasodilation and vasoconstriction which involving the downstream signal pathway of modulating vasodilation PKG/VASP (cGMP dependent protein kianse/vasodilator stimulated phosphoprotein). The present project plans to reveal in depth that SCU and it’s derivative inhibit VD induced by hyperglycemia combined with hyperlipidemia, adjust vasodilation and vasoconstriction via the upstream signal pathway NOS/GC/PKG (NO synthase/Guanylyl cyclase/PKG). Wire Myograph and molecular biology techniques are applied in the investigation at the levels of animal, isolated blood vessel, cell and molecular. Through a broader and further investigation, the present study might not only reveal novel insights about the molecular mechanisms of SCU and its derivative in protection against VD, but also provide evidence of the applications of SCU and its derivative in the prevention and treatment of VD.