肿瘤干细胞具有自我更新和多向分化能力，被认为是肿瘤复发转移的根源，但是环状RNA在肿瘤干细胞中的功能和机制目前尚不清楚。我们前期通过高通量RNA测序分析了骨肉瘤干细胞相关的环状RNA,从中筛选出环状RNA circ_0006633进行了初步研究。我们拟在对骨肉瘤干细胞和环状RNA前期研究的基础上，深入研究circ_0006633与骨肉瘤干细胞自我更新、侵袭、转移潜能的关系，探索circ_0006633在骨肉瘤干细胞中发挥功能的分子机制和表达调控机制，分析circ_0006633与骨肉瘤临床病理特征的关系及其临床意义，揭示circ_0006633在骨肉瘤发生发展过程中所起的作用，为骨肉瘤的诊断治疗提供新的思路。

Cancer stem cells with the ability of self-renewal and multi-directional differentiation are considered to be the source of cancer recurrence and metastasis. However, the function and mechanism of circRNA on cancer stem cells is unclear. In our previous study, we analyzed the circRNA associated with osteosarcoma stem cells by high-throughput RNA sequencing, and screened out the circRNA circ_0006633.In the present project, based on the previous research of osteosarcoma stem cells and circRNA, we will study effect of circ_0006633 on self-renewal, invasion and metastasis of osteosarcoma stem cells.Then we will investigate the molecular and regulation mechanism of circ_0006633 in osteosarcoma stem cells. Moreover, we will analyze the relationship between circ_0006633 and clinicopathologic features of osteosarcoma, and explore the role of circ_0006633 in the development and progression of osteosarcoma, which would provide new ideas for the diagnosis and treatment of osteosarcoma.