结直肠癌是威胁人类生命的常见恶性肿瘤之一。通过开发新型吻合技术，以进一步提高结直肠手术安全性，减少吻合口并发症，是值得积极探索的课题。本课题旨在阐明射频能量引导的肠道组织焊接技术在组织的焊接和热损伤形成中的微观机理，并解决活体动物肠道组织焊接的关键工程与技术问题。在离体组织和活体动物肠道组织焊接系列实验中，研究关键的生物物理学和电热学参数及其与射频组织焊接质量的相互关系；优化射频组织焊接能量平台及活体动物实验的相关器械设备；从宏观（热损伤范围及程度、爆破压、炭化及粘连度）和微观（病理组织切片、扫描电镜、蛋白质定量分析）角度，研究肠道吻合焊接的机理、吻合质量及存活动物的整体生存质量；并根据上述研究结果建立射频组织焊接过程中的热损伤控制方法。本课题将深入研究射频肠道组织焊接机理，建立组织焊接的理论、模型及质量评价体系，指导开发具有自主知识产权的电外科技术及设备。

Colorectal cancer is one of the most prevalent life-threatening malignancies. It is a challenge to improve the surgical safety in addition to qualified radical colorectal surgery and to develop a band-new technique to reduce the anastomotic events. The aim of the study is to clarify the microcosmic mechanism of radio-frequency (RF) induced tissue fusion of colorectal anastomosis as well as its thermal damage, and to solve the critical problems of engineering and technical issues on survival animal in vivo. By series of welding experiments on colorectal tissues in vitro or in vivo, we will explore the key biophysical and electrothermal parameters and their relationship with the quality of RF induced tissue fusion. We will optimize the technical platform for RF tissue welding and develop the required device for animal studies. We will investigate the mechanism of thermal welding and the quality of colorectal anastomosis, as well as the overall life quality of the survival animals from the different views, including those macroscopic assessment variables such as thermal spread and depth, bursting pressure, tissue charring and sticking levels, and those microscopic methods such as pathological section, scanning electron microscopy, protein analysis, ets,. Furthermore, we will establish the certain control methods for the thermal damage during RF induced tissue fusion according to these results. The study will go into mechanism of radio-frequency induced colorectal tissue fusion, and establish theory, model and quantity evaluation system for tissue fusion,which will own independent intellectual property in the future.