ω-3脂肪酸是一种市场需求量大的高值健康型脂肪酸，在大豆和油菜等普通油料作物种子中含量较低，而在“低投入、耐逆境、高产出”的新型油料作物亚麻荠种子中高达45%。为解析亚麻荠种子高水平合成积累ω-3脂肪酸独特分子机制，本项目以转录因子CsbZIP16和脂肪酸脱氢酶CsFAD3-2基因为靶标，用我们优化的亚麻荠高效遗传转化技术，培育CsbZIP16和CsFAD3-2超表达株系和CRISPR/case9介导基因敲出突变体，分析靶基因对种子油脂合成等表型的影响。通过酶联免疫和染色质免疫沉淀法检测CsbZIP16是否直接激活CsFAD3-2基因表达。用酵母单、双杂交检测与CsbZIP16互作蛋白。建立CsbZIP16介导ω-3脂肪酸生物合成及高水平积累的调控网络。研究将获得植物油脂合成调控新知识，拓展油脂代谢工程的理论和策略，推动这一高值珍稀脂肪酸的规模化绿色生产和可持续利用。

ω-3 fatty acid is one kind of high-value healthy fatty acids with large market demand. Common oil crops such as soybean and rape seed only contain small amount of ω-3 fatty acid while Camelina sativa, a new-type oilseed with “low input, stress tolerance and high output” features, could accumulate high up to 45% of the unusual fatty acid. For understanding the unique molecular mechanism underlying high biosynthesis and accumulation of ω-3 fatty acid in camelina seeds, transcription fatty CsbZIP16 and fatty acid desaturase CsFAD3-2 are selected as the target gene in the present study. To investigate their functions responsible for seed oil metabolism and other phenotypes in camelina, we will develop the transgenic seed-specifically expressing the two targets, respectively, and also the CRISPR/case9-mediated gene-knockout mutants by an effective genetic transformation method established in our lab. ELISA-based DNA binding assay and chromatin immunoprecipitation will be employed to test if CsbZIP16 can transactivate CsFAD3-2 expression in vivo and in vitro. Yeast-1-hybrid and yeast-2-hybrid will be used to identify proteins interacted with CsbZIP16. Finally, we will develop a model to elucidate CsbZIP16-mediated regulation network controlling biosynthesis and high accumulation of ω-3 fatty acid in camelina seeds. The present study will uncover new knowledge, enriching theory and practice of plant oil biosynthesis and metabolic engineering, and promoting large-scale green production and sustainable utilization of this high-value unusual fatty acid.