铀矿采冶过程中所产生的含铀废水，若不进行及时治理，将威胁人类健康，造成一定的环境安全问题。研究含铀废水治理新方法并实现铀的高效富集，对铀资源化和有害辐射防护均具有重要的战略意义。本项目组大量的前期研究，发现普通生物吸附剂对铀、氟等一些离子缺乏理想抗性，导致实际应用受限。针对目前含铀废水治理中的瓶颈问题，本项目拟采用CRISPR/Cas9基因编辑技术，将还原基因DsrA、耐氟基因flr，同时转入耐辐射奇球菌DR基因组中稳定表达，构建一种兼备高富集容量以及氟铀高耐受力等优势的高效富集双抗性功能基因工程菌。通过分析其生物学特性、耐辐射与氟的功能基因分析，采用实时荧光定量PCR、Solexa高通量测序以及基因转录技术研究其遗传特点、高效富集铀的行为与机理以及氟铀胁迫响应机制，为铀矿冶含铀废水微生物治理提供理论基础。本项目对指导铀厂矿安全生产和尾矿库辐射防护等方面具有重要的应用价值。

Wastewater containing Uranium would threaten human health and cause certain environmental security during the Uranium mining and Uranium hydrometallurgy process, if it was not treatmented in time. Therefore, studies on the new method for wastewater containing Uranium treatment, enriching Uranium high efficient from the wastewater containing low concentration of uranium, have important strategic significance for Uranium resource and harmful radiation protection. Based on special experimental installations and prophase research achievement of biological treating Uranium contaminated water in our school, many bio-adsorbents were lack of ideal resistance for some Uranium and fluorine ions, and limited to be used in actual application. So a novel double resistances function genetic engineering bacteria would be constructed, using DsrA、flr gene into Deinococcus radiodurans(DR) at the same time using CRISPR/Cas9 gene editing techniques, which is high enriching capacity, radiation resistance and better stress activity on fluorine and Uranium. The theory evidence of biology treating wastewater containing Uranium would be builded, which would be completed by analyzing the biological characteristics, the function genetic of the resistance to radiation and fluorine, and study on the genetic characteristics, the behavior and mechanism of enriching Uranium high efficient,and the response mechanism on fluorine and Uranium stress.At the same time, it would be supported to the safe produce in the Uranium mines and remediation of the entironment around tailings areas of uranium mines. This work is feasible with brilliant prospected and scientific significance.