自体脂肪作为填充材料常用于修复重建和器官再造，但因其移植后组织被大量吸收、坏死、纤维化等影响远期效果。早期能否及时建立有效血液循环是关键因素。我们前期研究显示，人参皂苷Rg1（G-Rg1）能增强脂肪干细胞（ASCs）增殖与定向分化功能；而特定基因修饰后的ASCs或细胞因子能有效地提高脂肪移植的成活率。本项目拟采用GFP标记人乳腺脂肪来源干细胞（HBASCs）；将不同浓度G-Rg1预处理HBASCs，观察G-Rg1对HBASCs增殖分化及旁分泌功能的影响；进而将自体脂肪颗粒与G-Rg1预处理后的HBASCs移植于裸鼠体内，观察移植物的成活与血管再生情况；通过抑制PI3K/Akt通路，探索该通路在G-Rg1促进HBASCs增殖分化过程中的分子机制及下游靶基因；进一步通过体内实验验证这些靶基因对自体脂肪移植的影响，为临床上提高自体脂肪移植成活率、减少移植后常见并发症及改善治疗效果提供理论依据。

Autologous fat itissue is commonly used as filler material for wounds repair and organ reconstruction, however, its long-term effect could be affected by absorption, necrosis and fibrosis of the fat transplants themselves. The fate of transplanted fat tissue depends on the adequacy of blood perfusion during the early stages after transplantation.Our previous studies have shown that ginsenoside Rg1 (G-Rg1) can enhance the proliferation and differentiation capacity of adipose-derived stem cells (ASCs), and the ASCs modified by specific genes or some cytokines can effectively improve the survival rate of fat transplantation. In this study, firstly,we intend to label human breast adipose-derived stem cells (HBASCs) with green fluorescent protein (GFP). Subsequently,we observe the effects of different concentration G-Rg1 on the proliferation, differentiation and paracrine function of HBASCs. Thirdly, we transplant the mixture of autologous fat and G-Rg1 pretreatmented HBASCs into nude mice to observe the survival and revascularization condition of fat grafts. Fourthly, through the inhibition of PI3K/Akt pathway, we explore the molecular mechanism of the PI3K/Akt pathways in the process of G-Rg1 promoted HBASCs proliferation differentiation, and downstream target genes. Lastly, we further verify how these target genes influence the autologus fat transplantation through vivo experiment. This study aims to provide theoretical basis of the improvement of autologus fat transplant survival, reduction of the common complication for transplantation in clinical application.