腹壁缺损修复至今尚无一种理想材料。在修复早期由于血供不足及组织需氧量增加导致缺损部位呈慢性缺氧状态，如何促进缺氧条件下植入材料的早期血管化是腹壁缺损再生修复的难题。我们前期研究发现三维打印适宜孔径的生物支架及外源性VEGF可促进植入材料血管新生，但存在模拟细胞外基质微环境不足、新生血管脆弱及无法维持VEGF的持续有效释放。定向诱导干细胞内皮分化能有效促进组织的血管新生，因此我们推测若能构建高度模拟细胞外微环境的仿生支架，通过调控干细胞定向分化为内皮细胞，将有可能实现缺氧条件下有效促进植入材料血管新生及腹壁再生修复的目的。本课题拟首先以PLLA为骨架构建具备足够力学强度的温敏凝胶仿生支架，通过对脂肪干细胞HIF-1α/VEGF表达进行调控并在仿生支架上行脂肪干细胞三维生物打印，研究定向诱导脂肪干细胞内皮分化对血管生成及促进腹壁再生修复的效果与机制。研究结果将为腹壁修复材料学研究提供重要指导。

There are no ideal materials for repairing abdominal wall defect up to now. The repair site presents a chronic state of hypoxia due to poor blood supply and increased tissue oxygen demand in the early stage of repair. How to promote the early vascularization of implanted materials under hypoxia is a difficult problem in the repair and regeneration of abdominal wall defects. In our previous study, we found that 3D printed bioscaffolds with appropriate pore size and exogenous VEGF can promote the angiogenesis of the implanted materials, but the simulated extracellular matrix microenvironment is insufficient together with the fragile neovascularization and the inefficient controlled releasing of VEGF. Targeted induction of stem cell endothelial differentiation can effectively promote the angiogenesis of tissue. Therefore, we speculated that if a biomimetic scaffold with a high degree of simulation of the extracellular microenvironment could be constructed, it would be possible to effectively promote angiogenesis and abdominal wall regeneration of the implanted materials under hypoxia conditions by regulating the directional differentiation of stem cells into endothelial cells. In this study, a thermosensitive gel biomimetic scaffold with sufficient mechanical strength is firstly constructed on the framework layer of PLLA to regulate the expression of HIF-1α /VEGF of adipose derived stem cells. And 3D bioprinting of adipose derived stem cells is performed on the biomimetic scaffold to study the effect and mechanism of the adipose derived stem cells’ endothelial differentiation on angiogenesis and promoting repair and regeneration of abdominal wall tissue. The study will significantly improve researches on implant material for abdominal wall defect repair and reconstruction.