软骨损伤是运动医学的常见病和多发病，严重困扰普通人群和运动员的日常生活和训练比赛，给社会经济和公众健康带来沉重的负担。本项目针对目前临床软骨一线治疗中存在的实际问题，组织工程软骨中种子细胞和支架材料缺乏靶向特异结合等难点。本研究拟在分离培养微创来源外周血MSC的基础上，通过噬菌体展示技术筛选外周血MSC特异亲和多肽P7，并以共价结合偶联至基于结构功能双重仿生设计的新型水凝胶Gtn-HPA/HA-EGCG表面，联合药物动员外周血和局部组织的大量内在自体MSC靶向特异募集归巢到缺损区，还在体外模拟体内软骨修复微环境观察MSC的靶向迁移，并在转录和表观遗传水平探索新型水凝胶结合P7特异募集MSC促进软骨再生的机制。本研究基于双重仿生和精准微创设计的外周血干细胞亲和肽P7结合新型水凝胶促进软骨原位再生，给临床软骨修复提供参考。

Articular cartilage injury is the common and frequently-occurring disease in orthopedic sports medicine. It is also a world problem faced by clinicians and researchers. It seriously plagues the daily life and competitive training of ordinary people and professional athletes, and brings a heavy burden to the social economy and public health. This project is aimed at the actual problems in the first-line treatment of clinical cartilage treatment. Seed cells and scaffold materials lack the specific targeting binding in tissue engineered cartilage. Based on the mobilization of peripheral blood MSC with minimally invasion, peripheral blood MSC specific affinity peptides P7 is screened by phage display technique and covalently coupled to the novel injectable hydrogel Gtn-HPA/HA-EGCG surface on structural and functional dual biomimetic design, which combined with drug mobilization of peripheral blood for autologous MSC targeted specific recruitment and cartilage repair in vitro and in vivo. And the mechanism is also explored at the transcriptional and epigenetic levels. Based on dual biomimetic design and precise minimally invasion, peripheral blood stem cell specific affinity peptides P7 combined with the novel hydrogel Gtn-HPA/HA-EGCG will provide a reference for in situ cartilage regeneration.