WOX11介导的活性氧（ROS）和蛋白质乙酰化修饰在水稻冠根发育中相互作用研究

根是植物非常重要的营养器官，与产量、品质密切相关。冠根是水稻根系特有的主要组成成分。因此，水稻冠根发育调控机制的研究具有十分重要的科学理论意义和生产实践价值。WOX11是WUSCHEL-related homeobox（WOX）家族基因，主要在水稻冠根起始与成熟根尖分生区表达。敲除或者超表达该基因都会造成冠根的异常发育。在最近的工作中，我们发现wox11突变体根中活性氧（ROS）水平和相关基因表达都发生了变化，这说明WOX11参与冠根中ROS水平的调控。同时，我们的研究结果也表明WOX11与SAGA（Spt-Ada-Gcn5-Acetyltransferase）复合体中的ADA2的互作招募组蛋白乙酰化酶GCN5改变了全基因组的乙酰化修饰水平。本研究的主要目的是解析WOX11、组蛋白乙酰化和ROS水平之间的关系，进一步完善水稻冠根发育的基因调控网络，为水稻分子育种提供理论指导。

Root is a very important vegetative organ of plants, which is closely related to plant yield and quality. As one of the most important food crops in the world, Crown roots are the main components of root system in rice. Therefore, it is of great scientific significance and practical value to study the regulation mechanism of crown root development. WOX11 is one of WUSCHEL-related homeobox (WOX) family genes. It is mainly expressed in the meristematic zone of crown root tip. Knock-out or overexpression of WOX11 transgenic plants display abnormal crown root phenotypes. In the recent work, we found that ROS (Reactive Oxygen Species) level and the expression of related genes was inhibited in wox11 mutants, which indicated that WOX11 was related to the regulation of ROS level in crown root. Meanwhile, we also found that WOX11 recruited histone acetylase GCN5 through interaction with ADA2, which is a component of SAGA (Spt-Ada-Gcn5-Acetyltransferase) complex, to alter the acetylation modification level genome wide, thus activating the genes expression. What is the relationship between WOX11, histone acetylation and ROS level during rice crown root development? We expect that this study will elucidate the interplay between the three, further improve the gene regulatory network of rice crown root development, and provide theoretical guidance for rice molecular breeding.