针对煤化工废水中大量存在的芳烃类有机物难以有效去除，导致废水生化处理系统不能稳定运行、出水难以达标排放的实际情况，聚焦芳烃类污染物开启生物转化的触发条件与驱动机制关键问题，实现芳烃类污染物在复杂开放环境中的高效、快速降解。本项目拟以煤化工废水中普遍存在的苯酚、萘、喹啉和苯并[a]芘为主要目标污染物，采用同位素示踪及分子生物学技术，通过对目标污染物微生物代谢归趋、关键酶系以及中间代谢产物的深度解析，明确目标污染物化学结构与其微生物降解有效性的内在耦合关系，并从关键酶基因转录表达水平、酶活性水平及微生物细胞响应水平等多层次揭示关键酶的诱导及目标污染物在共代谢体系中实现生物转化的驱动机制。在此基础上，构建多种芳烃类物质共存的微生物共代谢体系，提出基于代谢网络定量分析的共代谢调控策略与方法。研究成果将为煤化工废水的无害化处理及芳烃类污染物的生态修复提供理论指导与技术支持，具有重要的理论及实际意义。

Aiming at the actual situation that aromatic hydrocarbons in coal chemical wastewater are difficult to remove effectively, which lead to the unstable operation of wastewater biochemical treatment system and the difficulty of effluent discharge up to the standard, the project will focus on the key issues of triggering conditions and driving mechanism of aromatic hydrocarbon pollutants to initiate biotransformation, so as to achieve efficient and rapid degradation of aromatic hydrocarbon pollutants in complex open environment. In this study, phenol, naphthalene, quinoline and Benzo[a]pyrene, which are ubiquitous in coal chemical wastewater, will be taken as the main target pollutants. Isotope tracing and molecular biology techniques will be used to identify the internal coupling relationship between the chemical structure of aromatic hydrocarbons and their biodegradation efficiency by depth analysis of microbial metabolic trends, key enzymes and intermediate metabolites of target pollutants. In addition, the induction methods of key enzymes and the driving mechanism of target pollutants to achieve biological transformation in co-metabolic system will be revealed at multiple levels analysis of transcription and expression of key enzyme genes, enzyme activity and microbial cell response etc. On this basis, the microbial co-metabolism system under the coexistence of various types aromatic hydrocarbons will be constructed, and the strategies and methods of co-metabolism regulation based on quantitative analysis of metabolic network will be put forward. The research results will provide theoretical guidance and technical support for the harmless treatment of coal chemical wastewater, which has important theoretical and practical significance.