我国蕉园土壤有机碳含量和生物活性偏低，如何提升土壤有机碳含量和生物活性是香蕉产业绿色发展面临的重大挑战。项目利用覆盖作物多样性，提出“植物-微生物互作系统提升土壤剖面有机碳和生物活力”的研究思路。以广西集约化香蕉种植体系为研究对象，在田间通过深/浅根型覆盖作物混种，并配施有机肥，探究覆盖作物根系特征、根际微生物多样性和活性对蕉园土壤剖面有机碳固持过程的影响。项目利用核磁共振和傅里叶变换离子回旋共振质谱分析等技术,测定有机碳化学结构、可溶性有机碳的分子化学多样性；采用高通量测序探究土壤微生物多样性和群落结构，结合原位酶谱技术，分析参与碳循环的土壤酶活性；通过关联和网络分析，揭示根系分布特征和土壤微生物群落结构对土壤团聚体中碳固持的影响,在团聚体水平上理解覆盖作物介导的有机碳-微生物互作机制。本项目旨在探究蕉园酸性土壤剖面有机碳提升的生物学潜力和机制，为实现香蕉绿色种植提供重要理论支撑。

Soil organic carbon content (SOC) and biological activities of banana orchards are low in China. How to improve SOC and biological activities are major challenges for the sustainable development of banana industry. We propose a new conceptual strategy of using the interactive effects of cover crops and soil microbiome to promote SOC and living activities. The project is aimed to understand the mechanism of mixing deep/shallow-root cover crops combined with organic fertilizer to improve carbon sequestration along soil profiles in field experiments in Guangxi intensive banana orchards. NMR and FT-ICR MS will be used to determine the SOC chemical structure and molecular chemodiversity of dissolved organic matter respectively. The diversity and community structure of soil microbials are determined using high throughput sequencing. The activities of soil enzymes involved in the carbon cycle will be determined by in situ soil zymography. The effects of root distribution and microbial community structure on carbon sequestration will be determined through the correlation and network analysis. At soil aggregate level, this study will facilitate our understanding of the mechanisms by which cover crops mediate organic carbon-microbiome interactions. The objectives of the project are to explore the biological potential and mechanism of improving organic carbon in banana orchards in acid soils. The outcome of the project will provide theoretical supports for realizing the green development of banana orchards.