Toll抗病毒通路介导水稻条纹病毒持久侵染介体的机制研究

目前在介体传播植物病毒的研究中从未报道Toll抗病毒通路参与病毒的持久侵染。前期研究表明水稻条纹病毒（Rice stripe virus, RSV）激活介体Toll抗病毒通路，本项目拟在前期工作基础上，通过生物化学、细胞生物学、分子生物学和植物病毒学等多学科交叉手段系统地分析Toll抗病毒通路介导RSV持久侵染介体的分子机制。明确Toll蛋白识别RSV核衣壳蛋白的分子特性，探明转录因子Dorsal调控靶标蛋白表达的抗病毒机制，解析Toll抗病毒通路在病毒持久侵染介体中发挥的作用。本项目的实施有助于了解介体持久传播植物病毒的调控机制，同时也为综合防治虫传植物病毒提供理论依据。

Toll antiviral pathway has never been reported in the persistent infection of plant virus into insect vector. Our preliminary studies show that Rice stripe virus (RSV) activates the Toll antiviral pathway of vector. Based on this finding, this project intends to use the integrated approaches of biochemistry, cell biology, molecular biology and plant virology To analyze the molecular mechanism of Toll antiviral pathway mediated the persistent infection of RSV in vector. To clarify the molecular characteristics of the Toll protein recognition of RSV nucleocapsid protein, ascertain the antiviral mechanism of effector genes regulated by transcription factor Dorsal, and analyze the function of Toll antivirus pathway in the viral persistent infection of vector. The implementation of this project is helpful to understand the regulation mechanism of the persistent transmitted plant viruses by vector, and also provides a theoretical basis for the comprehensive control of insect-borne plant viruses.