BMP9促进髓核细胞维持软骨样特性的作用及机制研究

批准号:
81501922
项目类别:
青年科学基金项目
资助金额:
18.0 万元
负责人:
税巍
依托单位:
学科分类:
H0608.骨、关节、软组织退行性病变
结题年份:
2018
批准年份:
2015
项目状态:
已结题
项目参与者:
郭书权、倪卫东、梁安霖、罗刚、张晓军、刘佳渝、魏家华、娄钦
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中文摘要
退变性椎间盘疾病是老年人群中的常见疾病,髓核组织细胞外基质成分和结构的改变,尤其软骨样基质分泌的减少是椎间盘退变的重要机制。因此,通过外源性基因转染椎间盘髓核细胞,促进其软骨样特性的维持,从而延缓椎间盘的退变是目前研究的热点。其中骨形态发生蛋白(BMP)是非常具有临床应用前景的治疗因子。在课题组的前期研究中,初步发现BMP9可能具有促进髓核细胞合成和分泌软骨样基质的作用。本课题拟以兔髓核细胞为研究对象,通过重组腺病毒体系,提高胞内BMP9的表达水平,以软骨样基质Ⅱ型胶原、蛋白多糖等为检测对象,分析BMP9促进髓核细胞维持软骨样特性的作用;并深入研究其与Notch信号通路的关系,从分子水平明确BMP9维持髓核细胞软骨样特性的机制,为探寻BMP9作为一种治疗因子在椎间盘退变性疾病中的临床应用提供理论依据和实验基础。
英文摘要
Degenerative disc diseases are common in elder people. The essential mechanism for the degeneration of intervertebral disc is the change in the components and structure of the extracellular matrix of nucleus pulposus, especially the decrease of the secretion of chondroid matrix. Therefore, researches are now focusing on delaying intervertebral disc degeneration through exogenous gene transfection of nucleus pulposus cells to maintain its chondroid features. Bone morphogenetic protein (BMP) is a clinically promising therapeutic factor. In the preliminary studies of our research team, BMP9 was found to have significantly enhanced the synthesis and secretion of chondroid matrix of nucleus pulposus cells. In this study, we plan to intervene in the expression of BMP9 in the rabbit nucleus pulposus cells through recombinant adenovirus system, analyze the effect of BMP9 on the maintenance of chondroid phenotype of nucleus pulposus cells by measuring the type II collagen and proteoglycan, and study its relation with Notch signaling pathway to clarify the mechanism for BMP9 maintaining the chondroid feature of nucleus pulposus cells at the molecular level, providing theoretical and experimental basis for the clinical application of treating degenerated disc with BMP9.
专著列表
科研奖励列表
会议论文列表
专利列表
BMP9调节疼痛感受器TACAN的分布抑制机械压力疼痛的作用和机制研究
- 批准号:n/a
- 项目类别:省市级项目
- 资助金额:0.0万元
- 批准年份:2022
- 负责人:税巍
- 依托单位:
国内基金
海外基金
