肝细胞癌的转移侵袭是肝癌防治的重点与难点之一。我们前期研究表明鳖甲煎丸能有效调控wnt信号通路中的关键蛋白分子GSK-3β的表达水平而达到抗肝癌转移侵袭的目的。基于鳖甲煎丸抗肝癌研究的良好工作基础，以及miRNAs与肿瘤密切相关的研究进展，本研究拟以miRNAs差异表达调控GSK-3β为切入点，以不同转移潜能肝癌细胞株及裸鼠人肝细胞癌模型为研究对象，采用miRNAs芯片、质粒转染、荧光素酶、基因沉默、PCR等技术，首先高通量筛选与肝癌侵袭转移密切相关的差异表达的miRNAs，并探讨其对GSK-3β的调控作用，在此基础上进一步观察鳖甲煎丸对miRNAs差异表达的影响，深入研究鳖甲煎丸抗肝癌转移侵袭的分子机理。本研究对筛选肝细胞癌的诊断、治疗及预后的有效指标和寻找新的治疗靶点具有重要的临床意义。同时，有助于进一步诠释鳖甲煎丸多途径、多层次抗肝癌的科学内涵，为该药的临床应用提供充分的理论依据。

The metastasis and invasion of hepatocellular carcinoma is one of the key and difficult points of the prevention and treatment of liver cancer. Our preliminary study showed that the expression level of Biejiajian Pills could effectively regulate and control the expression of key protein molecule GSK-3β in the Wnt signaling pathway so as to achieve the purpose of anti-liver metastasis and invasion. Based on the good work of Biejiajian Pills in anti-liver cancer research and the research progress which miRNAs are closely related with tumor, miRNAs differential expression and regulation of GSK-3β is taken as the breakthrough point in this study. The different metastatic potential hepatoma cell lines and nude mice human hepatocellular carcinoma models are taken as the research object. We plan to high throughput screen out the diffiential expressed miRNAs using miRNAs chip, plasmid transfection,luciferase,gene silencing and PCR technology,et al and study the regulatory mechanism of miRNAs closely related with the invasion and metastasis of liver cancer targeting on GSK-3β. On this basis, we plan to observe the effect of Biejiajian Pills on the differential expression on miRNAs and making further discussion on the molecular mechanism of anti-liver cancer of Biejiajian Pills.Our study can be of important clinical significance to screen the effective diagnosis, treatment and prognosis indexes of hepatocellular carcinoma and to find new therapeutic targets as well as having great contribution in further elucidating the scientific connotation of the multi-channel and multi-level anti liver cancer of Biejiajian Pills which provides full theoretical basis for clinical application of Biejiajian Pills.