肝纤维化是多种慢性肝病发展至肝硬化的必经中间环节，其进一步发展将导致不可逆转的肝硬化，目前已成为世界范围内危害健康的重要问题之一。HSC活化、增殖是肝纤维化发生、发展的关键事件，本研究以HSC为靶标，以Wnt与NF-κB信号通路串话调控HSC活化、增殖为切入点，以鳖甲煎丸抗肝纤维化的分子机制研究为目标，拟从体内和体外两方面，以鼠肝星状细胞系HSC-T6和四氯化碳肝纤维化模型大鼠为研究对象，采用血清药理学方法及质粒转染、Western blot、RT-PCR、免疫组化、ELISA等技术，首先筛选出Wnt与NF-κB信号通路串话中与肝HSC活化、增殖相关的关键因子，在此基础上，再深入探讨鳖甲煎丸抗肝纤维化的作用机理。 本研究对深化和完善肝纤维化形成的基础理论研究具体十分重要的意义，同时有助进一步阐明鳖甲煎丸抗肝纤维化的作用靶点和途径，为该药应用于临床提供充分的理论依据。

As the intermediate link that many chronic liver disease must pass through to Irreversible cirrhosis,hepatic fibrosis has been one of the most serious health risk problems. Moreover,the activation and proliferation of HSC play a pivotal role in the happening and development of hepatic fibrosis.Our purpose is to study the molecular mechenism of anti-liver fibrosis of Biejiajian Pills by checking the effect of crosstalk between Wnt and NF-κB signal pathway in HSC activation and proliferation.We will use in-vitro and in-vivo experiments to study the mechanism choosing rat hepatic stellate cell and the carbon tetrachlorideinduced liver fibrosis modeI in rats as research object,respectively. We plan to screen out key factors related to HSC activation and proliferation of crosstalk between Wnt and NF-κB signal pathway using serum pharmacological method,plasmid transfection, Western blot,RT-PCR, immunohistochemistry, ELISA,et al. On the basis,making a further discussion of the effect of Biejiajian Pills on these factors.Our study can be of important significance in deepening the fundermental research of hepatic fibrogenesis as well as having great contribution in further elucidating the targets and routes of anti-liver fibrosis of Biejiajian Pills which provides full theory basis for clinical application of Biejiajian Pills.