基于微生物生态系统代谢网络构建的功能菌群对芘胁迫共适应机制研究

芘是典型的多环芳烃化合物（PAHs），是PAHs污染监测的指示物和研究其生物降解的模式污染物。细菌在芘污染环境的修复过程中起着重要作用。多种具有芘降解功能的细菌已被分离，其降解机制也得到了初步研究；而关于环境中功能菌群如何适应芘污染胁迫及它们之间的相互作用还需深入探讨。本研究拟借助微生物生态代谢网络的构建，揭示芘胁迫条件下功能菌群的共适应机制这一科学问题。拟通过定向驯化获得功能菌群，并对代谢产物分析重建功能菌群对芘的降解途径；借助高通量测序技术获取宏基因组信息，构建芘胁迫下的微生物生态代谢网络；基于纯培养功能菌的验证实验及功能基因表达的分析验证生态代谢网络稳定性；最后综合上述内容，揭示降解菌群之间、降解菌群与非降解菌群之间的相互作用，解析功能菌群共同参与芘降解的过程。研究结果将为理解功能菌群对芘污染胁迫的响应这一科学问题提供依据；并为通过生态代谢网络调节进行原位芘污染修复提供理论支持。

Pyrene, one of the important polycyclic aromatic hydrocarbons (PAHs), is regarded to be the indicator for measuring PAHs and also the model molecular for studying biodegradation of PAHs. Functional bacteria play a critical role in the bioremediation of pyrene. Even many bacteria capable of degrading pyrene have been isolated and their mechanism of degradation has been well studied, the interaction and co-acclimation of in situ bacterial consortium under the pollution of pyrene is far from well known. In this proposal, the microbial eco-system metabolic network for functional bacteria is going to be constructed to investigate the co-acclimation of bacterial consortium under the pollution of pyrene. Functional bacterial community and the pathway of pyrene degradation could be achieved by orient domestication; microbial eco-system metabolic network is expected to be constructed by analyzing meta-genomic information provided by High-throughput sequencing; the network is going to be verified by experiments based on cultured functional bacteria and mRNA; results will be integrated and analyzed to explain interactions between functional bacteria with degrading capability and between bacteria with and without degrading capability, and to interpret the co-biodegradation of pyrene by functional bacteria. This study will provide impressive data for revealing the co-acclimation of bacterial consortium under the pollution of pyrene, and provide a potential approach to remediate of pyrene by modulating microbial eco-system metabolic network.