益气活血中药调节冬眠心肌新生血管成熟的相关分子机制研究

冬眠心肌(hibernating myocardium,HM）是一种心肌防止自我坏死的保护状态。HM"进可复苏，退即坏死"。血管新生是促进HM复苏的重要基础，Ang/Tie2系统是调节新生血管稳定成熟的重要通路。以往研究表明益气活血中药能促进缺血心肌血管新生，但是否通过调节Ang/Tie2系统实现HM新生血管的成熟稳定，未见报道。本研究采用冠脉球囊扩张及内膜剥脱术制作小型猪慢性HM模型，以多普勒组织成像结合多巴酚丁胺负荷试验评价HM，运用双标免疫组化、激光共聚焦检测毛细血管密度、成熟血管指数和平均灌注指数，QCA方法观察冠脉侧支循环和HM组织灌注，Western Blot检测Ang1、Ang2和Tie2蛋白表达，RT-PCR检测Ang1、Ang2和Tie2基因表达。从病理、毛细血管、心肌灌注、蛋白表达、基因表达等多方面阐明益气活血中药通过调节Ang/Tie2系统实现HM心肌灌注的分子机制。

Hibernating myocardium (HM) is in a crucial self-protection condition which could prevent heart from myocardial infarction caused by chronic coronary ischemia. This condition maybe change to be recovery or infarction. New vascular maturation is an important mechanism that promote recovery of HM. Ang/Tie2 system regulates angiogenesis and vascular maturation. It need do a further work whether promoting angiogenesis of chinese herbal medicine for nourishing Qi and removing blood stasis is to make new vascular mature with regulation of Ang/Tie2 system. We use DTI-DSE technology to evaluate HM induced by balloon dilation and coronary endarterectomy in mini-swine model. And indexes of serum troponin T, serum VEGF, HIF-1α, collateral circulation, capillary density, mature vessels index, average flood fraction, Ang-1, Ang-2 and Tie-2 will be detected with these method such as ELISA, double standard immune histochemical method, laser scanning confocal microscope, RT-PCR, western blotting and so on. Thus we can expound the molecular mechanism of Ang/Tie2 system regulation to angiogenesis and vascular maturation of chronic HM.