开花期是影响大豆产量的重要农艺性状之一。SOC1（SUPPRESSOR OF OVEREXPRESSION OF CO1）是MADS-box转录因子家族的一员，在植物开花过程中起着重要的调控作用。本项目前期研究发现，通过CRISPR/Cas9技术敲除获得的大豆单突变体Gmsoc1a、Gmsoc1b和双突变体Gmsoc1a/Gmsoc1b在长日和短日照条件下都出现了晚花、成熟期延长及分枝数目增加的表型，表明GmSOC1a和GmSOC1b具有调控大豆开花的功能，且对产量具有潜在的影响，但是其参与的分子调控机制尚不清楚。因此，本研究拟利用酵母双杂交技术筛选与GmSOC1a和GmSOC1b互作的特异蛋白，并采用RNA-Seq、ChIP-Seq等分子生物学手段研究GmSOC1的下游调控网络，从而明确GmSOC1a和GmSOC1b调控大豆开花的具体生物学功能，解析其控制大豆开花的分子机理。

Flowering time is one of the key agronomic traits that directly affects soybean yield. SOC1 (SUPPRESSOR OF OVEREXPRESSION OF CO1) belongs to MADS-box transcription factor family and plays an important role in plant flowering. Our previous study revealed that, soybean CRISPR/Cas9-mediated knockout mutants Gmsoc1a, Gmsoc1b and Gmsoc1a/Gmsoc1b all exhibited later flowering and maturity and increased branch number under long-day and short-day conditions. These results indicated that GmSOC1a and GmSOC1b might be involved in the regulation of soybean flowering and may have the potential role on soybean yield. However, the underlying molecular mechanisms are still unclear. Therefore, in this study, yeast two-hybrid assay will be further used to screen specific proteins interacting with GmSOC1a and GmSOC1b; RNA-Seq, ChIP-Seq and other molecular biological methods will be performed to analyze the downstream regulatory networks of soybean GmSOC1. These analyses will deeply clarify the functions and molecular mechanisms of GmSOC1 in regulating soybean flowering.