恶性肿瘤细胞表面过渡表达蛋白的分离分析与癌症早期诊疗成正相关。但多数单一功能体系的肿瘤细胞表面过渡表达蛋白分离能力及复杂样品预处理能力易受高丰度蛋白影响及基质限制。先期研究发现，两亲性嵌段物具有较强功能可设计性及高丰度蛋白低吸附行为，因此采用可控合成法结合载肽识别体系的疏水端与可靶向降解的亲水端，装载于磁性材料表面，制备精准靶向分离材料。利用两亲性嵌段物肿瘤细胞微环境内的靶向降解，及内部多肽靶头的二次靶向，建立肿瘤细胞外表面过渡表达蛋白的高选择性分离方法。研究两亲性磁性纳米体系与恶性肿瘤细胞表面过渡表达蛋白的作用规律，开发靶向-富集、可控降解的样品预处理方法，构建可用于生物样品体系肿瘤细胞表面过渡表达蛋白的分离分析平台，以期实现癌症的早期诊断，为揭示恶性肿瘤表面行为变化与发展机制等提供依据与基础。

The accurately isolation and analysis of overexpression protein around tumor cell show positive correlation with the early diagnosis and treatment of cancer. The specific adsorption ability and pretreatment of complex samples are obstructed by high abundance protein and matrix, especially the adsorption system constructed with single functional carriers. These amphiprotic block copolymers are verified with designability functional and lower adsorption behavior towards high-abundance protein in our previous research. In hence, a controlled strategy is introduced in our work, and integrated the hydrophobic-hydrophilic system. The loading peptides parts and targeting degraded units are immobilized on the magnetic nanospheres. The amphiphilic magnetic system with strong dearation ability and anti-nonspecific adsorption are expected to reduce adsorption of obstacle and enhance targeting degraded effect around the pathological cells. In addition, the effect of degradation ability will exposed internal peptide which can afford secondary targeting and specific adsorption ability. The degradation ability, non-special adsorption and cellular uptake of amphiphilic block copolymer at different pH will be investigated, which will provide evidence for immediate effect a cell at pathological changes. Furthermore, the over expressed and pathological protein around the diseased cell will separate based on the degraded amphiphilic block copolymers effectively. This project offers a novel strategy for the construction of targeting and specific adsorption methods, as well as foundations of theory and practice for their applications in clinical diseases theranostic.