慢性乙肝严重危害人类健康，但其免疫相关机制未明。本项目在本课题组前期关于慢性乙肝的“遗传机制”的研究基础上，继续深入研究“免疫调控因子NLRX1及其编码基因变异（NLRX1 p.Arg707Cys）”影响HBV感染肝细胞能力的相关机制。本项目使用“慢病毒转染及过表达”、“蛋白表达纯化”等成熟技术，首先构建Huh7-NTCP细胞模型，以及NRLX1野生型过表达和NLRX1 p.Arg707Cys突变型过表达的Huh7-NTCP细胞模型，比较HBV感染状态的变化以明确其与HBV感染的关系；接着在预实验基础上，进一步明确NLRX1野生型和突变型是否会影响MAVS-RLRs介导的信号通路，影响I型干扰素和IL-6的表达，进而影响HBV感染肝细胞的能力；最终阐明NLRX1及其编码基因变异改变HBV感染肝细胞能力的机制，为最终明确HBV感染的免疫机制提供新的研究途径和基础。

Chronic Hepatitis B do serious harm to human health without immune mechanism been clarified. Based on our previous study about the genetic mechanism of HBV infection, this study is to discover the effects and mechanism of susceptibility to HBV infection in hepatocytes induced by immunoregulation factor NLRX1 and its encoding gene mutation NLRX1 p.Arg707Cys. Some well-handling techniques were used in this research including lentivirus transfection, gene over-express, protein expression and purification and so on. We will build Huh7-NTCP cell line, wild type NLRX1-overexpressing and p.Arg707Cys NLRX1-overexpressing Huh7-NTCP cell lines. And we want to investigate the effects on the the ability of HBV infection caused by NLRX1 changes in the cells. Then on the basis of preliminary experiment, we further investigate the influences of NLRX1 wild type and mutant in the MAVS- RLRs mediated signaling pathways, and the expression of type I interferon and IL - 6, and then affect HBV resistance ability of hepatocytes. We hope that the mechanism of NLRX1 and its encoding gene mutation to change the ability of HBV infection in hepatocytes could be finally revealed, which can provide basis and new strategy to the research of HBV infection immune mechanism.