基于ERK/CREB信号通路的慢性痛并发痛情绪的前扣带皮层-体感皮层关联机制及电针干预研究

疼痛定义赋予了疼痛以痛感觉和痛情绪双重含义。以往对慢性痛的研究多集中在痛感觉层面，在整体至分子水平取得了巨大进展，而对痛情绪知之甚少。研究者们逐步认识到对慢性痛发展过程中痛情绪的调节机制及有效干预措施的研究是目前疼痛研究领域急需解决的重大问题。本项目通过观察CFA致慢性炎性痛大鼠并复合体感皮层损毁后痛阈、痛情绪行为的改变、ACC脑区神经元放电和ERK/CREB蛋白表达的影响，探讨慢性痛并发痛情绪改变的ACC-体感皮层二维调制机制，初步明确电针干预痛情绪的ACC-体感皮层电活动双重调节作用。在此基础上，进一步观察慢性痛并发痛情绪改变大鼠ACC-体感皮层脑区ERK/CREB信号通路的蛋白表达变化并结合体感皮层ERK拮抗/激动剂微量注射实验，明确电针调节痛情绪的ACC-体感皮层脑区ERK/CREB信号通路分子机制。研究结果将赋予针刺镇痛新的科学内涵，并为针刺镇痛的临床应用提供新的理论依据。

Pain definition gives pain dual dimensions, which are pain sensation and pain affect. Past researches focused on pain sensation dimension, and made great progress in the levels from whole to molecule, but studies on pain affect are still lacking. Researchers are coming to realize that which are the regulatory mechanisms of pain affect and how to intervene effectively are the major problems to be solved urgently in the chronic pain processed. To answer these problems, the classic chronic pain model (CFA) of rats will be established by intra-plantar injection of complete freund’s ajuvant, combined with the lesion of somatosensory cortex. The paw withdraw thresholds, pain affective behaviour, neural spike and ERK/CREB protein expression in ACC will be detected，then to discuss the pain affect’s regulatory mechanism in dual dimensions and the invention of electroacupuncture (EA). Furthermore, the ERK/CREB molecular signal mechanism in ACC - somatosensory cortex of EA on pain affect will be determined, combined with the microinjection of ERK inhibitor and agonist to somatosensory cortex. This research project will reveal a new scientific significance of acupuncture analgesia, and provide a new insights and theoretical basis for clinical treatment of chronic pain.