ACC-IC神经环路调控痛厌恶记忆机制及电针干预研究

慢性疼痛是临床常见的难治性疾病，被认为与疼痛厌恶记忆密切相关。及早干预痛厌恶记忆成为可能有效的临床慢性痛治疗手段。课题组前期研究发现电针干预能有效缓解痛厌恶记忆的产生，故有必要深入探索针灸抗痛厌恶记忆的机制。研究显示ACC和IC是参与痛厌恶记忆的关键脑区，二者存在神经环路联系，并受MOR、GABAR等调控。由此提出假说：ACC-IC神经环路是调控痛厌恶记忆的关键神经环路，电针可能通过ACC-IC神经环路干预痛厌恶记忆。本研究采用角叉菜胶二次注射建立痛厌恶记忆模型，运用化学遗传法结合钙成像和免疫荧光法明确ACC-IC神经环路与痛厌恶记忆的相关性，联合药理学方法观察MOR、GABAR是否参与调控ACC-IC神经环路。在此基础上，探讨电针是否通过ACC-IC神经环路干预痛厌恶记忆及与MOR、GABAR的相关性。研究成果将充实中医针灸“既病防变”指导思想，为临床慢性痛针灸新疗法开发提供理论依据。

Chronic pain is a common clinical refractory disease, which is considered to be closely related with pain aversive memory. Early intervention in pain aversive memory may be an effective treatment on chronic pain. The research group found that electroacupuncture intervention could effectively mitigate pain aversive memory, so it is necessary to explore the new acupuncture treatment on pain aversive memory. Some researches show that anterior cingulate cortex and insular cortex are critical areas in pain aversive memory with existing neural circuitry between them and modulated by MOR and GABAR. We therefore put forward a hypothesis that ACC-IC circuitry is critical to regulate pain aversive memory, through which electroacupuncture could intervene in pain aversive memory. In this study, the pain memory model will be constructed by Second injection of carrageenin to the hind paws of rats. We will apply chemical genetic method combined calcium image and immunofluorescence assay to throw light on the correlation of ACC-IC circuitry condition to pain aversive memory, and then exploring if the ACC-IC circuitry was modulated by MOR and GABAR using combined pharmacologic methods. On these bases, we will explore if the electroacupuncture intervenes in pain aversive memory through ACC-IC circuitry and the correlation between MOR and GABAR. The results will enrich the Chinese medicine and acupuncture guidance of preventing disease from exacerbating and provide theoretical evidence for innovative acupuncture treatment on chronic pain.