铜绿假单胞菌是近50年来引起水貂出血性肺炎的主要病原菌之一，而水貂对该菌易感的机制未知。本研究在流行病学调查的基础上，选择代表菌株进行小鼠和水貂的致病性实验，筛选强毒和弱毒菌株。利用比较转录组学的方法，系统筛选引起水貂铜绿假单胞菌致病相关的主要差异表达基因，并构建差异基因的缺失突变株，进行动物致病性试验和其他相关毒力试验，研究差异基因与毒力的相关性。该研究可以部分阐明铜绿假单胞菌引起水貂出血性肺炎的分子机制，也为分子诊断和筛选药物靶点奠定基础。

Pseudomonas aeruginosa is one of the major pathogens of hemorrhagic pneumonia in mink for nearly 50 years, however, the mechanism of its susceptibility to mink remains unknown. On the basis of epidemiological investigation, representative strains will be chosen for pathogenicity tests to mice and mink to screen out the virulent and attenuated strains. Differentially expressed genes correlated with the pathogenicity of Pseudomonas aeruginosa from mink will be screened out by comparative transcriptomics. The gene deletion mutants will be constructed to study the pathogenicity to animals and other related experiments so as to know the relationship between the differentially expressed genes and their virulence. This research will partially elucidate the molecular mechanism of hemorrhagic pneumonia in mink caused by Pseudomonas aeruginosa and contribute for the molecular diagnosis and drug targets screening.