农药降解菌与生物材料纳米纤维素的有机结合，将为突破“降解菌应用活性的稳定”这个“瓶颈”难题提供新思路。申请人前期已成功制备降解菌球形节杆菌D47与纳米纤维素复合物，发现两者可协同促进除草剂敌草隆的生物降解，但协同作用的机理尚不清楚。基于环境中细菌倾向于黏附在固体表面形成生物膜应对外界胁迫的代谢方式，本项目以降解菌D47在纳米纤维素表面形成的生物膜为切入点，明确生物膜与敌草隆降解的相关性，研究纳米纤维素对生物膜形成以及生物膜内敌草隆水解酶PuhA活性的促进作用。通过解析降解菌利用纳米纤维素的结构优势，促进菌群增殖并提高酶促降解活性的途径，阐明降解菌与纳米纤维素促进敌草隆降解的协同作用机制，并优化制备高效、安全、经济的降解菌载体。预期结果将丰富农药环境学研究内容，促进解决农药降解菌实践应用核心技术难题，研究开发自主知识产权产品，为农药残留污染治理提供关键技术支撑。

The novel combination of pesticide-degrading microorganisms with the state-of-art biomaterial nanocellulose would provide a key breakthrough to tackle the bottleneck hindering the degradation stability of pesticide degraders in the practical applications. Previously, a hybrid nano-biocomposite was prepared by harnessing nanocellulose as a biocompatible scaffold for immobilizing the herbicide diuron-degrading bacterium Arthrobactor globiformi D47, which demonstrated efficient degradation of diuron with an obvious synergistic effect. However, the detailed mechanism of how bacteria and nanocellulose synergistically degraded diuron remained unclear. Since bacteria in the environment are prone to adhering to solid surface and forming biofilm in response to xenobiotic pressure, here, we attempt to investigate the mechanism of synergistic effect by exploiting bacterial biofilm formed on the surface of nanocellulose. First, the foundational correlation of diuron biodegradation efficiency along with bacterial biofilm formation is determined. Then, the improved biofilm formation and enhanced hydrolase PuhA-based enzymatic degradation inside the nanocellulose-supported biofilm architecture is further investigated. By elucidating the specific ways in which those pesticide degraders benefit from the structural advantages of nanocellulose to promote their proliferation and to enhance their enzymatic activity in degradation, the mechanism of synergistic degradation of diuron by strain D47 and nanocellulose is revealed. According to the unveiled mechanism, it would offer valuable guidance to optimize the preparation for a versatile, safe and economical carrier generally available for pesticide-degrading bacteria. In sum, the present study is expected to enrich the research contents in pesticide environmental science in theory, which would facilitate to solve the core technical problems that limit the practical application of pesticide-degrading microorganisms. More importantly, it provides vital technical support for developing products with independent intelligent property rights for the control of pesticide residual contamination.