猪繁殖与呼吸综合征病毒(PRRSV)引起免疫抑制的机制之一是抑制树突细胞(DC)的功能，但其确切机制尚不清楚。研究表明，自噬靶蛋白mTOR在DC免疫应答中发挥重要作用，而且PRRSV能通过诱导自噬促进自身增殖。本项目以此为切入点，研究mTOR在PRRSV感染中对DC的免疫调控。本研究拟以感染PRRSV猪外周血、骨髓、脾脏中分离的DC为研究对象，结合mTOR特异性抑制剂和激动剂，通过流式细胞术、ELISA等技术检测mDC/pDC亚群表型、亚群分化相关分子及Th1/Th2偏转情况，研究PRRSV通过mTOR对DC亚群分化的调控。采用RT-PCR、免疫印迹、荧光显微镜等方法测定抗原提呈、自噬相关基因和蛋白的水平，阐明PRRSV通过mTOR对DC功能的调控，从DC亚群失衡和DC抗原提呈紊乱的角度揭示PRRSV通过mTOR调控DC自噬水平来抑制DC功能的机理，从新的角度揭示PRRSV免疫抑制机制。

Porcine reproductive and respiratory syndrome virus (PRRSV) can inhibit the function of dendritic cell(DC),but the mechanism of immunosuppression has not been elucidated.Recent research showed that target protein mTOR of autophagy has an important role on immune response of DC.While,PRRSV can promote its proliferation by inducing autophagy. We had the research of mTOR immune regulation of DC during the infection of PRRSV based on the breakthrough point.The project intends to separate DC from peripheral blood,bone marrow and spleen with PRRSV infection as research model,binding specificity mTOR inhibitors and agonist.We will investigate the differentiation regulation of mTOR from mDC/pDC subtypes,differentiation related molecules and Th1/Th2 polarization by using techniques as ELISA,flow cytometry.And it will study the change of DC autophagy and the effect on antigen presentation from the gene and protein expression level by immunofluorescence,western blot and RT-PCR. It will identify dynamic regulation of mTOR from the subgroup of imbalances and immune dysfunction on DC. Thus it will reveal the immunosuppressive mechanism via autophagy level of DC by PRRSV and the pathogenic mechanism of PRRSV from a new perspective.