Pre-mRNA可变剪接是产生多细胞真核生物转录组和蛋白质组多样性的主要原因，也是细胞增殖、分化、凋亡以及个体发育等过程的重要调控方式。剪接位点的精确识别依赖于顺式序列元件、反式蛋白因子、表观遗传修饰等因素的协同作用。本项目以人类和拟南芥作为动植物基因组的模式生物，以外显子跳跃和内含子保留两类可变剪接事件为研究对象，利用生物信息学方法从RNA序列组分、内含子长度、序列模体、序列信息含量等一级序列信息，RNA回文结构、局部折叠等二级结构信息，核小体定位、组蛋白修饰、DNA甲基化等表观遗传信息三个维度系统地剖析动植物基因组间可变剪接调控机制的差异；发展区分动植物间同一类可变剪接事件的理论预测算法。该研究的顺利开展有助于真核基因组pre-mRNA可变剪接调控机制的解析，并为结合RNA序列、表观修饰信息、蛋白质相互作用等信息构建可变剪接调控网络提供依据，促进“剪接密码”的破译。

In multicellular eukaryotes, alternative pre-mRNA splicing is a major contributor to the expansion of the transcriptome and proteome diversity and is crucial for cell proliferation, differentiation, apoptosis, development, etc. The exact recognition of splice site is mainly determined by cis-acting RNA element, RNA structure, trans-acting protein, epigenetic information. In this project, the regulatory mechanism of exon skipping and intron retention in the human and A. thaliana genome is analyzed in terms of primary RNA sequence, secondary stucture and epigenetic information by using bioinformatics method. The information of primary RNA sequence is composed of sequence composition, intron length, sequence motif, and sequence information content. The information of secondary stucture is composed of palindrome structure and local folded structure. The epigenetic information is composed of nucleosome positioning, histone modification and DNA methylation. Furthermore, the regulatory differences of alternative splicing between aniamal and plant are elucidated. In addition, the theoretical algorithms are developed to classify the same type of alternative splicing events between aniamal and plant genome. This study will contribute to the understanding of regulatory mechanism of alternative splicing and provides some evidences for constructing alternative splicing regulatory network combining RNA sequence, epigenetic information, protein-protein interaction. In a word, the performing of this project plan will be helpful for deciphering the splicing code.