特定腐败菌（Specific Spoilage Organisms，SSO）是导致水产品腐败变质的主要原因，致病菌是导致食品安全的祸首之一。围绕贮藏流通过程中优势腐败菌的监控，研究开发更加安全、高效的新材料、新方法和新技术，代表着目前水产品保鲜及质量安全控制领域的研究重点和难点。本课题拟针对腐败希瓦氏菌和副溶血性弧菌等水产品中优势危害性微生物，采用琼脂渗透法和RP-HPLC分离纯化和鉴定出具有活性的鱼体表粘液抗菌肽,并利用蛋白质组学、透射电镜、16S rDNA DGGE等手段，通过测定目标微生物细胞膜结构和通透性、蛋白质合成、代谢活力、与微生物DNA相互作用等方面，探明粘液抗菌肽作用靶点，进一步阐明其抑菌作用途径及机制,同时将粘液抗菌肽应用于大黄鱼等水产品的保鲜，探究和验证其对水产品贮藏品质的影响。本研究旨在阐明鱼类体表粘液抗菌肽的抑菌作用机制，为下一步构建基因工程菌并工业化生产奠定基础。

The metabolic activity of specific spoilage organisms is the main reason for the aquatic spoilage, and the action of pathogenic bacteria is one main factor of food insecurity.Around the monitoring of specific spoilage microorganisms in the circulation process, the research and development of safer, more efficient new materials, methods and technology represent difficulty in the field of aquatic freshness and quality safety. The objectives of this project are to understand the preparation technology of the epidermal mucus antimicrobial peptides of fish, and to investigate the inhibitory effects to the Shewanella putrefaciens and Vibrio parahemolyticus, which are the main harmful microorganisms in aquatic products. Agar penetration and reversed-phase high performance liquid chromatography (RP-HPLC) are used to screen and indentify the fish mucus antimicrobial peptides, new means such as proteomics, transmission electron microscopy and 16S rDNA DGGE means are used to investigate the dynamic change of the target microbial cell membrane structure and permeability, the protein synthesis, the metabolic activity, and the genomic DNA interactions. Therefore, anti-microbial mechanisms of mucus extracts will be interpreted clearly. Meanwhile, the epidermal mucus antimicrobial peptides will be tested on aquatic products, such as large yellow croaker, to observe the effects of fish flesh quality influenced by this newly bio-preservatives. The purpose of this study is to clarify the inhibition mechanism of the epidermal mucus antimicrobial peptides of fish, and lay a theoretical foundation for the construction of gene engineering bacteria, as well as their industrial products.