膝骨性关节炎(Knee Osteoarthritis，KOA)是宁夏地区中老年人常见病，针砂马准灸（针砂灸）是回族群众广为接受的治疗KOA的方法，治疗机理不明确。uPA系统能激活MMP-1、13，MMP-1、13变化最能反映软骨基质的变化，两者与KOA发展关系密切。前期研究证实，针砂灸能降低兔KOA模型中MMP-1、13表达。因此设想针砂灸治疗KOA通过调控关节软骨基质中uPA系统，下调uPA及受体uPAR，上调PAI信号通路，改变uPA系统与MMPs的协调作用，本研究的目的为验证此假说。本课题拟完成：①针砂灸能延缓KOA软骨退变；②应用分子生物学技术明确针砂灸可激活uPA系统，抑制MMP-1、13的表达；③采用针砂血清干预模型兔软骨细胞，证明针砂血清中含有能抑制软骨退变的活性成分。通过以上研究，阐明针砂灸治疗KOA的机理，及针砂血清中含活性物质能够抑制uPA的表达而达到治疗KOA的目的。

Knee Osteoarthritis (KOA) is the common disease of the old people in the ningxia region, the zhenshamazhun moxibustion is one of the hui people widely accepted method of treatment of KOA, treatment mechanism is not clear. The uPA system can activate MMP 1, 13, MMP 1, 13 change can reflect the changes of cartilage matrix, those are most closely associated with development of KOA.Preliminary studies confirm that zhenshamazhun moxibustion sand lower MMP- 1、13 in the rabbit model of KOA.So imagine zhenshamazhun moxibustion treatment of KOA is through regulating the uPA in articular cartilage matrix system, cut the uPA uPAR and its receptors, raised PAI signaling pathways, change the uPA system and coordinating role between MMPs and other factors, the purpose of this study is to test this hypothesis.This project will complete the following stydy : (1) zhensha moxibustion can delay the KOA cartilage degeneration; (2)Using molecular biology techniques find : the zhensha moxibustion can cut the uPA, uPAR, raised PAI, inhibit the expression of MMP 1 and 13 in treatment KOA ;(3) Using zhensha moxibustion serum intervene rabbit chondrocytes has proved that there are some active ingredient in zhensha serum. Through the above study, to clarify the mechanism of zhenshamazhun moxibustion treatment of KOA, contain that the zhensha moxibustion can cut the uPA, uPAR, raised PAI, inhibit the expression of MMP 1 and 13 in treatment of KOA and the active substances in zhensha serum can inhibit the expression of uPA and achieve the goal to treat KOA.