膝骨性关节炎(Knee Osteoarthritis,KOA)是宁夏地区中老年人的常见病，温针灸是治疗KOA的常用疗法，其治疗机理尚不明确。我们前期临床研究证实其治疗KOA疗效确切，并发现温针灸可降低KOA患者血清IL-1、IL-6的水平。而IL-1、IL-6、TNF-a、iNOS、COX-2、MMP-1、MMP-13等细胞因子表达与MAPKs信号通路相关，JNK/SAPK信号通路是MAPKs中易被热敏及针刺所激活的主要信号通路，因此设想温针灸可下调JNK/SAPK信号通路治疗KOA。本课题拟对KOA兔模型行温针灸干预，用免疫组化、RT-PCR、Western blot等方法对其关节软骨、滑囊及关节液IL-1、IL-6、TNF-a、iNOS、COX-2、MMP-1、MMP-13、JNK/SAPK及mRNA表达水平进行检测。明确温针灸治疗KOA的机理是通过下调JNK/SAPK信号通路实现的。

Knee Osteoarthritis (KOA) is a common disease of the elderly in theNingxia region, warm acupuncture is commonly used therapies for the treatment ofKOA, its therapeutic mechanism is not clear. We pre-clinical studies confirm theefficacy treatment KOA and found that the temperature acupuncture can reduce thethe KOA serum IL-1, IL-6 levels. Cytokines IL-1, IL-6, TNF-a, iNOS and COX-2,MMP-1 and MMP-13 expression and MAPKs signal pathway, JNK / SAPKsignaling pathway MAPKs susceptible to thermal and needle the major signaling pathway activated by the thorns, so imagine warm acupuncture can down-regulateJNK / SAPK signaling pathway treatment KOA,. This subject intends to warm acupuncture intervention the KOA rabbit model line, using immunohistochemistry,RT-PCR, Western blot method, the articular cartilage, bursa and synovial fluid IL-1,IL-6, TNF-a, iNOS in and COX -2, MMP-1, MMP-13, of JNK / SAPK and mRNAexpression levels were detected. The clear warm the mechanism of acupuncturetreatment KOA is achieved by down-regulating JNK / SAPK signaling pathway.