由于在世界范围内缺少口腔鳞癌淋巴转移模型，导致人们对口腔颌面部鳞癌淋巴转移机制所知甚少。本课题组已建立了一对口腔鳞癌淋巴转移细胞系（GDC185和GDC185-1），拟以此为主，再选取Tca-83和Cal-27口腔鳞癌细胞系，即裸鼠舌部接种后，再从颌下转移淋巴结中培养出淋巴高转移细胞系，为研究口腔鳞癌淋巴转移机制提供研究基础。在研究口腔鳞癌淋巴转移机制方面，我们以GDC185和GDC185-1等口腔鳞癌淋巴高低转移细胞系为研究对象，拟采用划痕、侵袭实验、Real time PCR， Western Blot，免疫组化、细胞口腔原位种植-颌下淋巴结转移动物实验等手段，以VEGF-C和VEGF-D为突破口，同时辅以转录组学等高通量分析技术，研究VEGF-C和VEGF-D在口腔鳞癌淋巴转移中的作用及其调控机制。

Owing to the lack of lymphatic metastasis model of oral squamous cell carcinoma (OSCC) in the whole world, little is known about the underlying mechanism related to lymphatic metastasis of OSCC. GDC185-1 is a lymphatic metastatic OSCC cell line derived from submandible lymph node in nude mice that had been inoculated by GDC185 cells in the tongue in our lab. In this proposal, we are planing to establish new lymphatic metastasis OSCC cell lines by using Tca-83 and Cal-27 OSCC cell lines through tongue inoculation-submandible lymph nodes screening methods. We will take the lymphatic metastasis OSCC cell line pair, GDC185 and GDC185-1, and others that will be established as our models to investigate the uderlying mechanism of OSCC lymphatic metastasis by using wound healing assay, Transwell invasion assay, Real time PCR, Westrn blot, immunostaining, animal experiments via cell inoculation in the tongue tissue-submandible lymph nodes screening methods. The underlying mechanism will be focused on the roles of VEGF-C/VEGF-D in the process of OSCC lymphatic metastasis through the methods described above as well as highthrough approches such as digital gene expression.