抗菌肽广泛存在于各种生物体内，能够抵抗病原微生物入侵, 是生物体先天免疫的重要组成部分。抗菌肽不仅具有广谱抗菌活性和抗真菌、抗寄生虫、抗肿瘤细胞等生物活性, 还具结构的多样性，能够有选择的杀死特定的生物细胞，而对其他生物无毒副作用;它不易产生耐药性，是一类极具潜力的传统抗生素替代品。但是，目前抗菌肽作用机制的研究还有很多亟待解决的问题，尤其是抗菌肽与细胞膜的相互作用细节仍不清楚。本项目拟选取几种分别抗革兰氏阳性菌、革兰阴氏性菌和真菌等病原体的抗菌肽，应用nanodisc 技术和liposome 来模拟细胞膜体系，以核磁共振为主要的研究手段，结合荧光、电镜等其他辅助手段，研究抗菌肽与细胞膜的相互作用的动态过程及其在细胞膜体系存在情况下的溶液结构，并探索抗菌肽与细胞膜的相互作用的分子机制，为阐明抗菌肽杀灭微生物的机理提供重要的理论依据。

Antimicrobial peptides are very important peptide molecule, which exist widely in living nature. Antimicrobial peptides are key components of the innate immune system of most multicellular organisms which can defend against pathogeny microbe. Antimicrobial peptides are potential drug instead of antibiotic based on they have very different antimicrobial mechanism without drug tolerance. Antimicrobial peptides have the broad spectrum antibacterial activity and are the potent antimicrobial activities against bacteria, fungi，protozoa and cancer cell. Antimicrobial pipetides have structural diversity and have selectivity to target cell, which can kill microbe and be safety for multicellular organisms. But, right now, the detail interaction between antimicrobial peptides and cell membrane is still not clear, and many questions about the effect mechanism of antimicrobial peptides must be answered. So, in this proposal we select several antimicrobial peptides against Gram positive bacteria, Gram negative bacteria and fungi, respectively. We will mainly rely on NMR technique, combining fluorescence and electron microscope, and make nanodisc and liposome to mimic cell membrane, and apply it study the interaction between antimicrobial peptides and cell membrane, solution structure of antimicrobial peptides with cell membrane. The research will explore the molecular mechanism of interaction between antimicrobial peptides and cell membrane and elucidate the mechanism of antimicrobial peptides how to kill microbe.