健脾补肾方干预辐射损伤小鼠wnt细胞信号通路的表达

中医认为"健脾补肾"与机体的造血和免疫功能关系密切。以此为法则组建"健脾补肾方"，该方对辐射损伤具有保护作用，前期临床试验表明该方能提高外周血象及增强免疫功能，前期动物实验从骨髓干细胞的生成、骨髓及脾脏相关凋亡基因表达及脾脏组织病理学改变等方面阐述了补肾健脾方对小鼠辐射损伤保护作用的机制。在血液系统疾病的研究中已发现Wnt信号通路存在翻译异常，可知细胞信号通路的某些异常信号表达可导致造血异常。本课题组考虑在前期工作基础上，拟采用PCR及western方法检测骨髓干细胞Wnt信号通路的异常信号表达，从细胞信号转导角度阐明健脾补肾方对化学损伤保护作用的机制，为新药研发提供理论依据。

Chinese medicine believes that the invigorating spleen and kidney is close to the body's hematopoietic and immune function.This law is the rule to formate the invigorating spleen and kidney preciption,the preciption has a protective effect on the radiation damage,pre-clinical trials showed that in order to improve the peripheral blood and enhancedimmune function,early animal experiments to generate stem cells from bone marrow,bone marrow and spleen related apoptosis gene expression and the spleen of thehistopathological changes described the mechanism of injury protection effect in kidney and spleen prescription on the mouse radiation.Wnt signaling pathway has been foundtranslation abnormalities in the study of the blood system disease,we can see the expression of some of the abnormal signal in the cell signaling pathways can lead to abnormal hematopoiesis.Research group consider based on previous work,and intend to use PCR and westerm methods to detect the expression of abnormal signal of Wnt signaling pathway of bone marrow sten cells,and clear the mechanisms of protective effect on invigorating spleen and kidney prescription to chemical injury from the angle of the cell signaling,in order to provide theoretical basis for new drug discovery.