乳腺癌转移导致乳腺癌难治和复发。表皮生长因子受体通路底物8(Eps8)促进乳腺癌转移，是预防乳腺癌转移和根治乳腺癌的新靶点。我们已在前期研究中成功构建了Eps8-CRT-chi9558疫苗，并在小鼠荷4T1乳腺癌模型中初步显示出良好的抗肿瘤免疫效应，但其抗乳腺癌转移的作用及机制亟待进一步阐明。因此，本课题设计以Eps8为靶点切入，复制小鼠移植乳腺癌和自发乳腺癌模型，通过荧光活体成像等体内动物实验明确Eps8-CRT-chi9558抗肿瘤生长及转移的效果；体外免疫学实验分析复合疫苗对抗肿瘤体液免疫和细胞免疫的影响；基因芯片和Westernblot评价复合疫苗对肿瘤转移相关信号通路中基因和蛋白表达的作用，可望阐明Eps8这一新靶点疫苗在防治乳腺癌转移中的作用及其机制，为该疫苗用于临床预防和治疗乳腺癌转移提供实验依据，为根治乳腺癌提供新方法和策略。

Tumor metastasis is the leading cause of refractory or relapsed breast cancer. Epidermal growth factor receptor pathway substrate No.8 (Eps8) facilitates metastasis of breast cancer and represents an attractive target for preventing and treating metastasis of breast cancer. In our previous studies, we have successfully constructed Eps8-CRT-chi9558 vaccine and it preliminarily demonstrated satisfactory anti-tumor effect on murine breast cancer model. However, further elucidation of the mechanism is urgently needed. In this study, we plan to target at Eps8 and establish transplanted and spontaneous murine breast cancer models. Moreover, in vivo optical imaging animal experiments will be performed to elucidate the anti-growth-and-metastasis effect of Eps8-CRT-chi9558 vaccine against breast cancer. Furthermore, we will examine humoral and celluar immune responses induced by the combined vaccine in anti-tumor immunity, and will evaluate gene and protein expression in anti-metastasis-related signaling pathways influenced by the combined vaccine by gene chip and Westernblot in vitro. Taken together, our study tries to unveil the anti-metastasis effect and mechanism of the novel Eps8-targeted vaccine against breast cancer. These findings will estabilish an experimental basis for preventing and treating metastasis of breast cancer, and thus will provide a new method and strategy for radical treatment of breast cancer.