间质性膀胱炎/膀胱疼痛综合征（IC/BPS）的疼痛症状严重影响患者的生理及心理状态。既往研究表明，瞬时感受器电位家族（TRPs）是介导膀胱疼痛传入的重要通路，但单一阻断该家族受体无法有效改善疼痛。TRPM8是一种同时存在于神经元和非神经元中的受体，既往报道：IC/BPS患者膀胱中TRPM8高表达；外周神经及DRG神经元上TRPM8高表达会降低患者感觉神经疼痛阈值；我们初步证实，TRPM8在IC/BPS患者及动物模型的膀胱中高表达；但尚未证实膀胱与DRG中的TRPM8高表达之间是否相关。本课题拟利用野生型、TRPM8基因过表达及基因敲除小鼠IC/BPS疾病模型结合患者标本，验证TRPM8在IC/BPS膀胱组织及DRG中高表达是否相关，明确TRPM8在IC/BPS患者膀胱-外周神经-DRG这一整条感觉神经传导通路中的作用及与此类疼痛发生、发展的关系，为进一步研究新型疼痛抑制治疗方法提供线索。

The clinical symptoms of interstitial cystitis/bladder pain syndrome (IC/BPS) seriously affect the physiological and psychological functioning of IC/BPS patients. Previous reports showed that the transient receptor potentials (TRPs) could be a key pathway in mediating bladder pain afferent, although the blocking of the receptor alone can not improve the painful symptoms. TRPM8, a receptor presented in both neuronal and non-neuronal tissues, was found to be increasingly expressed in IC/BPS patients' bladder. Meanwhile, higher TRPM8 level in DRG neuron could decrease the pain perception threshold. Our preliminary study identified an increased expression of TRPM8 in bladder tissues from either IC/BPS subjects or animal models. However, no relation of higher TRPM8 expression in the bladder to the DRG has been explored. In this study, we will use wild type, TRPM8 gene over-expressive or gene knock-out IC/BPS mice models combined with human specimens to verify the relationship between increased expression of TRPM8 in the bladder and the DRG neuron of IC/BPS. Our aim is to confirm the role of TRPM8 in the whole sensory afferent nerve including bladder-peripheral nerve-DRG neuron, as well as the effects of TRPM8 on painful symptoms of IC/BPS so as to provide evidence for new therapeutic strategy in pain management.