线粒体自噬是细胞依靠溶酶体选择性降解损伤线粒体，促进细胞生存的过程。近年来，医学研究者发现了pink1-parkin通路是介导线粒体自噬参与神经、心脑等疾病发生发展的重要机制，然而其与慢性肝损伤的关系尚不明确。右归丸是中医临床常用的温阳复方，具有良好的促细胞生存作用，能改善慢性肝损伤，但缺乏明确的分子机制。本项目把小鼠肝实质细胞内线粒体自噬作为观察对象，在体外以过氧化氢造成线粒体损伤，采用shRNA-plasmid转染技术截断pink1-parkin通路；在体内则利用高脂饲料造成慢性肝损伤，采用氯喹阻断线粒体自噬流，体外体内相结合动态观察线粒体自噬的变化情况，期望明确慢性肝损伤下，pink1-parkin通路是调节肝实质细胞内线粒体自噬的关键机制。同时设立右归丸干预对照组，观察肝实质细胞损伤时，右归丸对pink1-parkin通路介导的线粒体自噬的影响，探索右归丸改善慢性肝损伤的分子机制。

Mitophagy is a process that cells specially degrade damaged mitochondrion through lysosomes to promote cell survive. Recently, medicine investigators revealed that the pink1-parkin pathway, which mediating mitophagy, is involved in various diseases, such as neurogenic disease, heart and brain disease, etc. However, the relationship between pink1-parkin pathway and chronic liver injure is unclear. You-Gui pill is a ingredient usually used to warm deficiency of Yang in Chinese medicine clinic. Pharmacology researches showed that You-Gui pill was able to promote the cell survive or improve chronic liver injure while there is no distinct molecular mechanism. This project plans to take mitophagy in mouse hepatic cell line as the observed object. In vitro, hydrogen peroxide is used to induce mitochondrion damaged, and then the shRNA plasmid is used to knockdown parkin gene, which could block the pink1-parkin pathway. In vivo, the project will use high fat to cause chronic liver injury and then make use of the chloroquine to block mitophagy. By observing the changes of mitophagy both in vitro and vivo， the project wants to reveal that pink1-parkin pathway should be the key mechanism of mitophagy in chronic injured hepatic cells. At the same time, the project will set up You-Gui pill treatment groups to investigate the effects of You-Gui pill on the Pink1-parkin pathway mediating mitophagy in the injured hepatic cells, and try to discover the molecular mechanism of You-Gui Pill improving chronic liver injure.