提高哺乳动物成体中枢神经损伤再生能力一直是神经科学领域的研究热点。神经元轴突损伤后生长锥的动态重构和轴突的快速推进过程与微管骨架的动态重构关系密切，是影响损伤轴突再生的重要环节。我们发现微管依赖的驱动蛋白Kinesin-12和微丝依赖的马达蛋白Myosin-IIB互作形成复合物，通过联系微管-微丝影响细胞皮质区骨架的动态性调节细胞行为；前期实验表明二者在生长锥转换区存在共定位；下调Kinesin-12表达可减小生长锥面积和丝状伪足数量，促进轴突延伸；由此推测该复合物在生长锥动态重构中发挥调节作用。本项目拟采用斑马鱼模型对此复合物在神经发生和轴突损伤再生过程中的作用机制进行在体研究，动态观察其在生长锥导向过程及损伤轴突顶端生长锥动态重构中的作用，考量此复合物缺失对生长锥形态、轴突长度和分枝等动态重构因素的影响，进而通过磷酸化位点突变与超分辨率成像等实验阐明该复合物影响生长锥动态的分子机制。

To enhance the regenerative ability of the central nervous system is always a hotspot in neuroscience. Microtubule cytoskeleton dynamics is essential to the post-injury growth cone dynamic reconstruction and the rapid extension of axon in neuronal regeneration. We found that Kinesin-12，a member of the kinesin superfamily of the microtubule associated molecular motors, interacted and formed a complex with Myosin-IIB, a microfilament-based motor, and regulate cell behavior by associating the microtubule and microfilament cytoskeletons and acting on the dynamics of the cytoskeleton in the transition zone of the growth cone. Our experiment results suggested that these two motor proteins were colocalized in the transition zone of the growth cone and that downregulated expression of kinesin-12 lead to decreased growth cone size and quantity of filopodia, and promote axon extension. Hence, we infer the complex plays an important role in the dynamic reconstruction of the growth cone. In the present study, we shall conduct a research focused on the function of said complex in neurogenesis and post-injury axon regeneration in vivo utilizing zebrafish as a model animal. Initially, we will perform live imaging to observe the function of said complex in the path-finding of the growth cone and the dynamic reconstruction in the growth cone of an injured neuron. Secondly, examine dynamic reconstruction factors including growth cone morphology, axon length and branching in the absence of said complex. Finally, we will dig into the mechanism of its function utilizing point mutation experiments and ultra-resolution microscopy.