缺血性卒中有着较高的患病率及病死率。目前缺乏临床实用性的血清生物标记物和新的治疗方法。微小RNA(microRNA, miR)是一类存在于多种生物体中可调控基因表达的内源性非编码小分子RNA。miR-29b最近被认为与急性缺血性脑卒中有关，但是具体的机制未明。本研究拟通过建立缺氧/复氧损伤的细胞损伤模型和急性脑缺血动物模型，采用抑制或激动miR-29b的手段，明确miR-29b参与缺血性脑卒中的机制是否与其调节细神经细胞凋亡有关，并且在临床上分析血清miR-29b水平，与缺血性脑卒中患者预后，神经功能缺损和脑细胞代谢的关系。本项目的意义在于进一步阐明缺血性脑卒中的发病机制，寻找新的治疗靶点和生物标记物，为将miR-29b作为诊断，治疗和预后评估指标提供依据。

The prevalence and mortality of cerebral ischemic stroke are increasing rapidly worldwide. Economic serum biomarkers are lacking in clinic. MicroRNA is a kind of endogenous small non coding RNA molecules which excited in multi-organism and regulated gene expression. miR-29b has been suggested to correlate with acute cerebral ischemic stroke, but the underline mechanism remains elusive. In the present study, we are to determine the effect of miR-29b on neuron apoptosis. Hypoxia reoxygenation model in cells and acute cerebral ischemic animal are the main pathological models. We will use both in vitro and in vivo experiments coming with inhibitor and agonist of miR-29b for the detrimental roles of miR-29b on neuron apoptosis. We will further to analysis the relation of the serum level of miR-29b and the prognosis, neuron defect and cerebral metabolism in patients with cerebral ischemic stroke. This study would provide new insight into the mechanism of cerebral ischemic stroke and novel treatment and serum biomarkers for cerebral ischemic stroke. miR-29b might be used as the biomarkers for diagnosis, therapy and prognosis assessment.