即使在氧气充足的情况下，肿瘤细胞仍主要以糖酵解途径提供能量，这种现象被称为有氧糖酵解,是肿瘤的重要特征。肝动脉灌注化疗栓塞术(TACE)是中晚期原发性肝癌(HCC)的首选治疗方法，但远期生存效果并不理想，其原因可能由于TACE术后引起肿瘤出现缺血坏死，形成缺氧微环境所致。而缺氧微环境可引起缺氧诱导因子1 表达增加，增强肿瘤的糖酵解 ，促进肿瘤细胞的恶性转化。因此TACE术后HCC的复发转移可能与术后肿瘤的糖酵解增强有关 。本研究采用[U-13C]-glucose同位素追踪化疗药物表柔比星处理人正常肝细胞(L-02)和人 肝癌细胞系(HepG2、HuH7、MHCC97H)后缺氧诱导的糖酵解代谢变化，进一步在动物肝癌模型中注入[U-13C]-glucose同位素研究TACE术后缺氧微环境对细胞糖代谢的影响，探索肝癌TACE 术后肝癌复发转移的机制，为提高HCC患者TACE疗效提供依据。

One of the important hallmarks of tumor cells is the metabolic reprogramming, where the tumor cells metabolize glucose even in the presence of abundant oxygen , widely referred to as the aerobic glycolysis. Transarterial chemoembolization (TACE) is an important treatment for intermediated or advanced hepatocellular carcinoma (HCC), but the long-term efficacy of the procedure is not satisfactory. The major factor limiting its therapeutic efficacy is hypoxia microenvironment after TACE. Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor that mediates adaptive cell response to hypoxia, and it can favor the aerobic glycolysis promoting the tumor malignant transformation. To demonstrate the possible mechanism of the Warburg effect in accelerating the recurrence and metastasis of HCC cells, we use [U-13C]-glucose to compare glucose metabolism between HCC cells HepG2、HuH7、MHCC97H and hepatocyte line L-02. Futher more,we infuse [U-13C]-glucose to HCC models on rabbits, and analysis the glucose metabolism, the invasion and metastasis of tumor after TACE. So the implementation of this project is important clinical significance to explore the mechanism of the recurrence and metastasis after TACE and improve the efficacy of TACE for patients with HCC.