云南是肝脏片形吸虫流行的自然疫源地有着沉重的疾病负担，由于缺乏可靠的早期诊断和干预评估方法，往往使治疗延误和病情加重。前期临床研究发现肝片形吸虫感染，在易感性和治疗反应性存在明显个体间差异，细胞免疫因素可能是决定疾病感染早期结局的关键因素。目前在肝片形吸虫感染早期免疫变化和炎症反应关系未见研究报道，在影像改变和肝组织损伤程度间也缺乏连续动态的研究。通过建立SD大鼠肝片吸虫感染模型，在感染早期动态监测炎症指标、细胞免疫变化、组织损伤与影像变化的关系，探讨宿主细胞免疫与个体易感性、早期感染中Th1/Th2免疫偏移与炎症反应、影像变化及病理损伤的关系，以及抗虫药物三氯苯哒唑干预后对机体免疫的影响，探讨肝脏片形吸虫早期感染与个体易感性、早期感染的细胞免疫机制，细胞免疫与炎症反应，早期影像特征和病理损伤程度，研究将为进一步探索肝片吸虫感染的早期诊断和干预评价提供实验依据。

Yunnan province is the highest epidemic area of foodborne parasitic liver disease with a heavy burden of disease, becauese of the lack of early reliable diagnosis and treatment assessment methods, so always makes treatment delays and exacerbations. Preclinical studies have found that there are significant differences in susceptibility and therapeutic response in Fasciola hepatica infection, and cellular immune factors may be a key factor in determining the early outcome of disease infection. There was also a lack of continuous assessable index of early immunization, inflammation, image change and liver injury in the pathogen of Fasciola hepatica infection. To establish a rat model of Fasciola hepatica infection, to monitor the relationship between inflammatory markers, cellular immunity and image changes in the early stage of infection, to explore the relationship between host cell immunity and individual susceptibility and relationship between the Th1 / Th2 immune migration with inflammation infection , image changes and pathological damage in early infection, As well as the effect of the anti-insect drug trichlorobenzazole on the immunity of the body,to discuss the early infection of Fasciola hepatica, individual susceptibility , cellular immune mechanism of early infection, cellular immune and inflammatory response, the early imaging features and the degree of pathological damage, through the experimental basis providing a method to early diagnosis and assessment of Fasciola hepatica infection.