植物在免疫防御过程中主要通过控制细胞内氧化还原和表观遗传修饰如DNA甲基化和组蛋白修饰应对病原体的入侵，然而作为组蛋白修饰方式之一的组蛋白乙酰化与提高抗病性之间的机制仍不清楚。我们研究发现糖酵解酶OsGAPC1作为一种新的OsRac1蛋白相互作用因子和一氧化氮（NO）受体在水稻对稻瘟病菌的抗性中起到了正调控作用，其中组蛋白去乙酰化酶HDT701对OsGAPC1的乙酰化是整个通路的关键步骤；此外脱乙酰酶在过表达HDT701基因的情况下可以增强植物的耐盐及耐旱能力。基于以上发现我们将继续深入探讨OsGAPC1-HDT701复合物在不同胁迫条件下感受NO信号来调节组蛋白乙酰化进而影响植物免疫防御的分子机制，找出该复合物中与影响靶基因特定启动子区域乙酰化程度的功能域，进一步从三维结构上明晰该复合物在分子水平上的功能活性基础，为最终破译植物如何通过组蛋白的乙酰化应对病原体侵害的研究提供更多理论依据。

Plants control redox signals and epigenetic states to orchestrate their immunity to contend with pathogen invasion. Epigenetic marks like DNA methylation and histone modification reconstitute specific defense transcription circuits, which rapidly trigger host pathogen resistance. Nevertheless, it is as yet unknown how plants control histone acetylation during defense. .We have recently clarified the mechanism by which plants control histone acetylation to trigger fungal pathogen resistance. It was previously revealed that GTPase OsRac1 is a key regulator in rice immune responses. Preliminary data showed that the glycolytic enzyme OsGAPC1 participates in rice blast resistance and interacts with OsRac1. In rice immune responses, OsGAPC1 acts as a nitric oxide (NO) sensor that controls histone acetylation by regulating histone deacetylase HDT701. The present study indicates that the OsRac1-NO-OsGAPC1-HDT701 signaling module links a pattern recognition receptor and histone acetylation during defense. .NO contributes to various physiological processes and stress responses. Several stress conditions trigger the nuclear translocation of the glycolytic enzyme GAPDH. It has also been shown that the overexpression of deacetylase HDT701 enhances salt and drought tolerance. Based on this evidence, it is possible that the OsGAPC1-HDT701 module is a key stress tolerance regulator. The main objective of this project was to determine whether the OsGAPC1-HDT701 signaling module acts as a NO sensor to regulate histone acetylation under various stress conditions. In addition, three other objectives were proposed to validate the central hypothesis..The first goal was to understand the molecular mechanisms of OsGAPC1-HDT701 module-dependent immunity. The components of the OsGAPC1-HDT701 module are to be identified. An explanation will be sought for the mechanism by which this module regulates histone acetylation in the promoter regions of specific target genes during defense. Second, an attempt will be made to elucidate the three-dimensional structure of the OsGAPC1-HDT701 module and understand how it is activated. Third, it will be determined whether the OsGAPC1-HDT701 module is involved in the tolerance abiotic stress like drought, heat, and cold.