花生条纹病毒(Peanut stripe virus，PStV)属于Potyvirus，是我国花生上危害最严重的一种病毒，广泛流行于北方花生产区，寄主何种成分参与了PStV的致病过程却研究甚少。申请者首次获得了花生过氧化氢酶(Catalase, CAT)基因全长，通过酵母双杂交技术初步确定了CAT与PStV HC-Pro存在互作。基于上述结果，本研究以花生和PStV为主要研究材料，利用PStV病毒中HC-Pro蛋白及与植物抵御生物胁迫相关的过氧化氢酶基因家族互作的关联性作为研究切入点，明确抗感品种在不同生长时期以及PStV侵染后CAT表达量的变化，通过双分子荧光互补和酵母双杂交技术深入解析CAT和PStV HC-Pro在PStV致病过程中的关联性，并利用RNAi技术和过量表达技术详细验证CAT在PStV致病过程中的具体功能。本研究对深入揭示PStV致病机理和寄主抗性机制具有重要理论意义。

Peanut stripe virus (PStV) belongs to the Potyvirus, it is the most serious virus on peanut and widely spread in Northern China. There is little reseach on peanut factors involved in the pathogenic process. We first cloned the full-length of Catalase (CAT), Yeast two-hybrid system (Y2H) showed that there were interactions between CAT and PStV HC-Pro. Based on these studies, we proposed to use peanut and PStV as study material, focus on the relevance between biological stress resistance factor CAT and PStV HC-Pro, investigate CAT genes expression during different growing stages and after the PStV infection. Further analysis the interaction between CAT and PStV HC-pro by Y2H and bimolecular fluorescence complementation (BiFC). Silenced the CAT genes by VIGS and over-expressed CAT to study the relationship between peanut resistance of PStV and the regulatory role of CAT. This study has a significance to reveal pathogenic mechanism of PStV and resistance mechanism peanut, provide theoretical basic for peanut molecular breeding.