Proteomic Analysis of Histone Crotonylation Suggests Diverse Functions in Myzus persicae.
Proteomic Analysis of Histone Crotonylation Suggests Diverse Functions in Myzus persicae.
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组蛋白巴豆酰化的蛋白质组学分析表明桃蚜具有多种功能
DOI：
10.1021/acsomega.1c01194
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发表时间：
2021-06-29
期刊：
影响因子：
4.1
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Xu M;Xie Y;Li Y;Shen L;Huang K;Lin Z;Li B;Xia C;Zhang X;Chi Y;Zhang B;Yang J
桃蚜是重要的农作物经济害虫之一。在本研究中，我们使用了一个综合的方法，包括高效液相色谱分离，亲和富集，和质谱为基础的蛋白质组学进行全面的蛋白质组学分析的赖氨酸巴豆酸在M。桃蚜。总共在2452个蛋白质组中鉴定了7530个赖氨酸巴豆酰化位点。通过Gene Ontology注释、结构域注释、亚细胞定位、京都基因与基因组百科全书（Encyclopedia of Genes and Genomes pathway）注释、功能聚类分析等方法对赖氨酸巴豆酰化的靶蛋白进行了深入的生物信息学分析。核糖体等等。值得注意的是，相互作用网络表明，有199个巴豆酰化蛋白参与氨基酸代谢和许多巴豆酰化目标与脂肪酸的生物合成和降解。结果提供了整个M. persicae crotonylome和丰富的数据集，为这一重要经济害虫的巴豆酰化蛋白的功能分析，这标志着一个重要的开端，为进一步的研究。
Myzus persicae is one of the most important economic pests of cultivated crops. In the present study, we used an integrated approach involving high-performance liquid chromatography fractionation, affinity enrichment, and mass spectrometry-based proteomics to carry out a comprehensive proteomic analysis of lysine crotonylation in M. persicae. Altogether, 7530 lysine crotonylation sites were identified in 2452 protein groups. Intensive bioinformatic analyses were then carried out to annotate those lysine crotonylated targets identified in terms of Gene Ontology annotation, domain annotation, subcellular localization, Kyoto Encyclopedia of Genes and Genomes pathway annotation, functional cluster analysis, etc. Analysis results showed that lysine-crotonylated proteins were involved in many biological processes, such as the amino acid metabolism, aminoacyl-tRNA biosynthesis, spliceosomes, ribosomes, and so forth. Notably, the interaction network showed that there were 199 crotonylated proteins involved in the amino acid metabolism and numerous crotonylation targets associated with fatty acid biosynthesis and degradation. The results provide a system-wide view of the entire M. persicae crotonylome and a rich data set for functional analysis of crotonylated proteins in this economically important pest, which marks an important beginning for the further research.
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DOI：
10.1111/nph.12218
发表时间：
2013-06
期刊：
The New phytologist
影响因子：
--
作者：
Kettles GJ;Drurey C;Schoonbeek HJ;Maule AJ;Hogenhout SA
通讯作者：
Hogenhout SA
影响因子：
4.6
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Yang J
影响因子：
1.6
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Chen W;Tang D;Xu Y;Zou Y;Sui W;Dai Y;Diao H
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影响因子：
1.9
作者：
Mandrioli, M.;Zanetti, E.;Manicardi, G. C.
通讯作者：
Manicardi, G. C.
DOI：
10.1038/nrm.2016.140
发表时间：
2017-02
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Sabari BR;Zhang D;Allis CD;Zhao Y
通讯作者：
Zhao Y