牙周膜三维构象的形成与再生研究

牙周膜再生研究是当今研究的热点。完整的、功能性的牙周膜再生需建立在牙周膜三维网络结构被充分认知的基础上，但到目前为止，人们对该结构及其形成过程仍不清楚。现在一般认为牙周膜以纤维束的形式存在，但也有学者发现其为片状纤维结构。二次谐波显微成像是观察富含胶原纤维组织的先进技术，但尚未见用其观察出牙周膜三维结构的报道。本研究以牙周膜三维网络结构及形成机制为研究中心，首先用二次谐波显微镜观察三维组态，并动态观察该结构的发育过程。在细胞层面上，本实验拟利用牙周膜干细胞建立牙周膜再生模型，在干细胞被绿色荧光蛋白标记后，研究在组态再生过程中干细胞的行为、生长模式及分化状态。本项目从发育生物学出发，从组织及细胞层面探索牙周膜网络三维结构的形成及再生机制，为牙周组织再生技术提供重要理论基础。

The regeneration of periodontal ligament (PDL) is the hot field. Complete and functional PDL regeneration should be based on the knowledge of the 3-D structure of PDL. However, the structure and the pattern formation process are not clear. It is generally believed that PDL exists as fiber bundles. However, some one finds that the PDL collagen network is organized in sheets. The second harmonic imaging microscopy (SHIM) is the advanced tool to observe the tissues abundant with collagen fibers. However, the 3-D structure of PDL has not been observed clearly with this tool. The main purpose of this research is to study the 3-D structure of the fiber network and the mechanism of its pattern formation. First, we propose to observe the 3-D structure and the formation process of PDL with SHIM. At the cellular level, we will first create the PDL regeneration model with periodontal ligament stem cells (PDLSCs), then we will observe the cell behaviour, growth pattern, and differentiation situation of PDLSCs in the pattern regeneration process. From the aspect of development biology, the 3-D pattern formation and regeneration process of PDL will be studied in this research. The conclusions will be the fundamental theoretical basis for the regeneration technology of PDL.