SCAPs Regulate Differentiation of DFSCs During Tooth Root Development in Swine.
SCAPs Regulate Differentiation of DFSCs During Tooth Root Development in Swine.
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SCAPs 调节猪牙根发育过程中 DFSCs 的分化
DOI：
10.7150/ijms.22495
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发表时间：
2018
影响因子：
3.6
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Wu X;Hu L;Li Y;Li Y;Wang F;Ma P;Wang J;Zhang C;Jiang C;Wang S
牙根通过牙周组织向牙槽骨传递和平衡咬合力。牙周组织，包括牙龈、牙周韧带、牙骨质和部分牙槽骨，除牙龈外，均来源于牙囊。在早期发育阶段，DF像球体一样围绕在牙胚周围，起到促进牙齿萌发的作用。然而，在根伸长过程中，调控DF分化的形态动力学和因素仍然很大程度上是未知的。小型猪在牙列和下颌解剖方面与人类相似，被认为是颅面研究中有用的实验动物。本研究以小型猪第三乳中切牙为模型，探讨了牙根发育过程中影响牙周组织分化的因素。我们发现，牙周膜呈新月形，位于根尖和牙槽骨之间。Wnt5A、β-catenin和Col-I的表达水平从牙周膜中心(在根尖孔下)逐渐增加到牙周膜向牙周组织分化的外冠角。为了确定牙尖乳头对DF细胞分化的潜在调节作用，我们将牙囊干细胞(DFSCs)与根尖乳头干细胞(SCAPs)进行共培养。与SCAPs共培养时，DFSCs的成骨和成纤维能力均受到抑制，提示DFSCs的命运可受根尖乳头信号的调控。在根发育完成之前，顶端乳头可能维持DFSCs的未分化状态。这些数据深入了解了根尖和牙周组织在牙根和牙周组织发育过程中的相互作用，并为未来大型哺乳动物牙根再生的研究提供了线索。
The tooth root transmits and balances occlusal forces through the periodontium to the alveolar bone. The periodontium, including the gingiva, the periodontal ligament, the cementum and the partial alveolar bone, derives from the dental follicle (DF), except for the gingiva. In the early developmental stages, the DF surrounds the tooth germ as a sphere and functions to promote tooth eruption. However, the morphological dynamics and factors regulating the differentiation of the DF during root elongation remain largely unknown. Miniature pigs are regarded as a useful experimental animal for modeling in craniofacial research because they are similar to humans with respect to dentition and mandible anatomy. In the present study, we used the third deciduous incisor of miniature pig as the model to investigate the factors influencing DF differentiation during root development. We found that the DF was shaped like a crescent and was located between the root apical and the alveolar bone. The expression levels of WNT5a, β-Catenin, and COL-I gradually increased from the center of the DF (beneath the apical foramen) to the lateral coronal corner, where the DF differentiates into the periodontium. To determine the potential regulatory role of the apical papilla on DF cell differentiation, we co-cultured dental follicle stem cells (DFSCs) with stem cells of the apical papilla (SCAPs). The osteogenesis and fibrogenesis abilities of DFSCs were inhibited when being co-cultured with SCAPs, suggesting that the fate of the DF can be regulated by signals from the apical papilla. The apical papilla may sustain the undifferentiated status of DFSCs before root development finishes. These data yield insight into the interaction between the root apex and surrounding DF tissues in root and periodontium development and shed light on the future study of root regeneration in large mammals.
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