ARNT/HIF-1β是AhR和HIF-1信号通路的枢纽蛋白，两条通路均和ARNT/HIF-1β结合后激活下游基因的表达。采用实时定量PCR和 Western blot法检测AhR信号通路的CYP1A1、CYP1B1和HIF-1信号通路的VEGF 、CAⅨ的mRNA和蛋白水平的表达,研究BaP对AhR和HIF-1信号通路的影响。采用免疫沉淀法和Western blot法，探讨AhR和HIF-1α与ARNT结合情况，阐明两条信号通路的相互作用。采用免疫组化法检测被动吸烟对小鼠肺组织CYP1A1、CYP1B1、VEGF和CAⅨ基因的表达的影响。检测内蒙古地区蒙、汉族肺癌患者的癌组织和癌旁正常组织中CYP1A1、CYP1B1、VEGF和CAⅨ基因的表达，分析表达是否存在民族差异。从不同层次阐明BaP对ARNT/HIF-1β介导的AhR和HIF-1信号通路的影响及这些基因表达与肺癌发生发展的关系。

ARNT/HIF-1βis a pivot protein in the AhR and HIF-1 signal pathways. AhR and HIF-1αcombined with ARNT/HIF-1βand which activated the expression of CYP1A1,CYP1B1 and VEGF,CAⅨ genes. The methods of Real-time quantitative PCR and Western blot were used to assay the expression of mRNA and protein of CYP1A1, CYP1B1 and VEGF, CAⅨ genes. The influence of BaP on the AhR and HIF-1 signal pathways were studied by estimate the level of expression of CYP1A1,CYP1B1,VEGF and CAⅨ genes in normal rat trachea-bronchial epithelial cells and human lung carcinoma cells(A549). The methods of immunoprecipitation and Western blot were used to determine the degree of HIF-1αand AhR-bound ARNT in normal rat trachea-brochial epithelial cells and A549 cells. The cross talk of AhR and HIF-1 signal pathways were studied by estimate the degree of HIF-1α and AhR-bound ARNT. To know the influence of passive smoking to gene expression of CYP1A1,CYP1B1,VEGF and CAⅨ, the expression of CYP1A1, CYP1B1, VEGF and CAⅨ genes of passive smoking mouse lung tissues were determined by immunohistochemical staining methods. The difference of expression of CYP1A1,CYP1B1,VEGF and CAⅨ genes of lung cancer lesions and paracancerous tissues of Mongolia nationality and Han nationality people in Inner Mongolia Autonomous Region were determined by immunohistochemical staining methods. These results were also used to assay if there were nationality difference in the expression of CYP1A1,CYP1B1,VEGF and CAⅨ genes.The influence of BaP on AhR and HIF-1 signal pathways which were mediated by ARNT/HIF-1βwere illuminated on the level of genes, cells,tissues and bodies. The relationship of expression of CYP1A1, CYP1B1, VEGF and CAⅨ with the initiation and progression of lung cancer was clarified also.