摘要. 由于天然草地资源的限制及羊肉市场需求的增加，舍饲育肥成为了绒山羊羊肉生产的主要方式，由此引起的羊肉品质降低成为了制约羊肉生产健康发展的瓶颈问题之一。羊肉脂肪酸（FA）组成是影响肉产品品质的主要因素之一。项目以舍饲育肥的内蒙古白绒山羊为研究对象，根据肌肉组织FA组成变化揭示棕榈油与亚麻油混合添加对羊肉FA组成的改善效果是否优于单独添加，阐明可优化羊肉FA组成的主要FA种类；采用代谢组学、微生物多样性分析和实时定量PCR分析技术探讨瘤胃内差异显著的标志性FA种类、代谢过程及关键微生物，结合肌肉组织中FA代谢相关酶活性、基因与蛋白质的表达，从细胞、分子和代谢水平系统地研究棕榈油与亚麻油的混合添加对绒山羊羊肉FA组成具有优化作用的调节机理。研究结果为在生产中合理使用植物油脂调控山羊的脂类代谢、有效改善羊肉FA组成进而改进羊肉品质提供理论依据。

As a result of the limitation of natural grassland resources, the limit of grazing and grazing prohibition policy implementation and the increasing demand of mutton, stall feeding becomes one of the main producing ways for mutton production,leading to bad mutton quality,which is a problem of restricting the healthy development of mutton production.The experimental animals of this project are Inner Mongolia white cashmere goats experiencing satll feeding.Whether the improving effect of fatty acids in mutton by feeding the blend of palm oil and linseed oil is better than feeding palm oil or linseed oil alone can be revealed and the main fatty acids which could improve fatty acids composition of mutton can be clarifed according to the changes of fatty acids in muscle tissues as well as fattening performance and meat quality of white cashmere goats. By using the analyse of metabonomics ,microbe diversity in rumen and RT PCR to disscuss the main fatty acids,metabolic pathway and important microbes which have a large difference in rumen. And combining with analysing the enzyme activities, genes and proteins expression related to the fatty acid mechanism to systematically study the regulation mechanism of feeding the blend of palm oil and linseed oil in modifying fatty acid profile from the cellular, molecular and metabolic pathway. The results of the research provides theoretical basis for utilizing plant oils to reasonably regulate fat metabolism and effectively improve fatty acids composition in mutton, thereby improving mutton quality. All this findings have an important theoretical and practical significance to improve goats heath under stall feeding mode,increase meat performance and produce the mutton that are benefit to humen health.