肿瘤干细胞是癌症发生、发展的主要动因,也是癌症治疗三大难点--复发,转移及抗药性产生的主要载体。 我们的前期研究已经在国内外率先分离鉴定出宫颈癌干细胞; 证实转录抑制因子DAX1和EZH2能够促进宫颈癌细胞的增殖和肿瘤形成; 并发现DAX1和EZH2能够交互促进彼此蛋白的表达。本项目拟研究：(1)DAX1对宫颈癌干细胞功能的影响及分子机制；（2）EZH2对宫颈癌干细胞功能的影响及分子机制；（3）DAX1和EZH2在宫颈癌及胚胎干细胞中相互作用的分子机制；（4）DAX1和EZH2的相互作用对宫颈癌干细胞功能的影响及分子机制。通过上述研究，我们拟阐明DAX1和EZH2在宫颈癌干细胞中相互作用的分子机制，为靶向宫颈癌干细胞从转录抑制因子方面探寻新靶点。

Cancer stem cells (CSCs) are crucial in the initiation, development and progression of various human carcinomas，and also important in cancer metastasis, reoccurance, and drug resistance. Our previous studies showed that human cervical cancer stem cells had been firstly isolated and identified; and found that transcriptative suppressors,both DAX1 and EZH2 which might enhance the protein expression for each other, could promote the cervical carcinogenesis and development, respectively. In the present project, we plan to study: (1) DAX1 functions and molecular mechanism in cervcal cancer stem cells, (2) EZH2 functions and molecular mechanism in cervcal cancer stem cells, (3) Molecular mechanism of interaction between DAX1 and EZH2 in cervical cancer and human embryonic stem cells,(4) Molecular mechanism of interaction between DAX1 and EZH2 in cervical cancer stem cells. Through all above studies, we attempt to clarify the role by which DAX1 and EZH2 cooperate to function in cervical cancer stem cells. Results from such studies will provide new inofmration from the area of transcription repressors, such as DAX1 and EZH2, to develop novel stratigies to intervene cervical cancer targeting cervical cancer stem cells.