稻曲病菌优势真菌病毒对寄主菌致病力分化机制研究

稻曲病菌致病力分化是抗性育种中抗稻曲病材料筛选和鉴定的关键，真菌病毒是稻曲病菌中常见的生物因子，是否参与其生长发育和致病力分化的研究未见报道。本研究利用小分子RNAs深度测序技术，挖掘稻曲病菌的新真菌病毒，通过RT-PCR和旁侧序列RACE技术验证组装基因组，研究稻曲病菌真菌病毒的遗传多样性和优势种群，阐明优势种群对稻曲病菌生物学特性的影响；同时结合农杆菌介导遗传转化获得稻曲病菌突变体库进行致病相关基因的初步定位，研究真菌病毒对稻曲病菌致病力分化的影响和分子调控机制，为稻曲病抗病育种和真菌病毒源杀菌剂的挖掘提供理论依据。

Differentiation of pathogenicity on ustilaginoidea virens strains as one of key factors, which manipulates screening and identification resistant rice materials to rice false smut in the experiments, mycoviruses as one of important biological factors hosting in ustilaginoidea virens, it was a pity how to regulate the development and pathogenicity differentiation of its host have still been poorly understood yet. Small RNAs originated from different ustilaginoidea virens strains will be isolated and sequenced by deep sequencing, thus different mycoviruses as well as the relative genomes will be obtained and verified through assembling all sequences from RT-PCR, flanking sequences from 5'-RACE and 3'-RACE, respectively. Therefore, genetic diversity and structure of mycoviruses in ustilaginoidea virens in China will be further characterized; simultaneously the effect on biological characterization in ustilaginoidea virens by dominant mycoviruses will be elucidated. Under agrobacterium-mediated transformation, the relative fragments within 10kb preliminary mapped are responsible for pathogenicity of ustilaginoidea virens through its mutants with GFP gene insertion, when as the targets dominant mycoviruses how to regulate pathogenicity differentiation in host fungi strains will be displayed, as well as the molecular mechanism in the study. Take overall, there are expanding basic knowledge for contributing to resistance breeding and also developing novel fungicides origin from mycoviruses to rice false smut on rice production.