Fe元素在病菌生长发育和致病过程中发挥重要作用，目前关于Fe参与植物病菌致病机制研究鲜见报道。项目组前期发现辣椒胶孢炭疽病菌（Colletotrichum gloeosporioides）CFEM效应子CgCFEM2和CgCFEM10分别在活体和死体寄生阶段特异表达，并对Fe（III）敏感，但其互作机制和参与效应子致病机理仍不清楚。为此，项目拟在前期研究基础上，采用基因重组、生物化学和分子生物学等技术手段，解析CgCFEM2和CgCFEM10的致病机理；明确Fe参与CgCFEM2和CgCFEM10致病过程的作用；阐明CgCFEM2和CgCFEM10获得Fe的途径；揭示CgCFEM2和CgCFEM10与Fe结合的互作方式和结合位点。研究结果以期从营养免疫调控角度阐明CFEM效应子的致病机制，验证Fe参与效应子的生物学功能，为研发新型Fe螯合剂或阻断致病效应子Fe高亲和性化学药物提供科学依据。

Iron an indispensable element, is play an important role on regulating vegetative development and pathogenic development for many pathogens from human and plants. Secreted effectors as research hotspots are still being concerned in mechanism of plant pathogenic fungi. However, it is still unknown how iron homeostasis was occurred during pathogenic development of effectors protein. Pepper anthracnose caused by Colletotrichum gloeosporioides (C. gloeosporioides), is an important limiting factor during pepper production. Common in fungal extracellular membranes (CFEM) effectors are used as determinants factors for interaction between host plants and its pathogens, two CFEM effectors CgCFEM2 and CgCFEM10 have been confirmed to sensitive to ferric iron in our experiments, which were detected exclusive expression in biotrophic phase and necrotrophic phase, respectively. Interestingly, the relationship and response mechanism between iron homeostasis and pathogenic mechanism of effectors is still not elucidated . Therefore, on basis of our primary results, here the pathogenic mechanism will be elucidated on CgCFEM2 and CgCFEM10, respectively; iron attention how to regulate CFMEcg2 and CgCFEM10 pathogenic process will be confirmed; simultaneously, the pathway of iron obtained by CgCFEM2 and CgCFEM10 will be cleared as well, naturally their binding sites and interaction framework of iron and CgCFEM2\CgCFEM10 will be revealed in this study. Taking all, in the virtue of interdisciplinary perspective, our expected results will be helped to explain the pathogenic mechanism of CgCFEM2 and CFEM10 as well as iron biological roles during their pathogenic process. It is promising to develop iron chelating agent or chemicals of high affinity to iron to interfere with pathogenic effectors on the theory,. Finally, relative scientific proofs also will be used to improveand perfect to control fungal diseases.